The main methods and results are as follows: 1.The constructed expression vector, pMSCV-ncam, was constructed and proved correct by enzyme restriction pattern. 2.

• 实验方法结果如下： 1.；对已构建含有NCAM的质粒载体进行酶切鉴定，证明表达载体构建正确，转化入细菌(TOP10)，扩增，纯化，备用。