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- Methods The restriction endonuclease and T4 DNA ligase were used to construct the vector plasmid. 方法载体的构建采用限制性内切酶酶切、4DNA连接酶连接等方法。
- It was cloned into the vector of pET-28a to construct the recombinant plasmid pETLG1-3.The recombinant E. coli BL21(DE3)/pETLG1-3 was induced by IPTG. 用IPTG诱导重组大肠杆菌BL21(DE3)/pETLG1-3表达,SDS-PAGE分析表明在相对分子质量60000处有特异性重组蛋白表达条带,并经Western印迹进一步鉴定。
- OBJECTIVE:To construct the vectors, pDsVEGF165Red1-N1 and pIRES2-BMP2-enhanced green fluorescent protein(EGFP) followed by co-transfected into HEK 293-T cells,and study their expression and location of VEGF165 and BMP2 in the cells. 目的:构建在真核细胞中表达人血管内皮生长因子165的红色荧光蛋白表达载体和携带人骨形态发生蛋白2的绿色荧光蛋白表达载体,共转染真核细胞以研究血管内皮生长因子165和骨形态发生蛋白2在293-T细胞内的表达和定位。
- The vector product is said to be anticommutative. 可以说矢积是反交换的。
- Initializes a new instance of the Vector structure. 初始化Vector结构的新实例。
- First you have to construct the plot of the novel. 首先你得把小说的情节设计好。
- They are constructing the building at full blast. 他们倾全力建造这栋大楼。
- Next, they started to construct the main canal. 然后他们开始修建主渠。
- It took them two years to construct the bridge. 他们用了两年时间建这座桥。
- Material to construct the glider's rudder control. 滑翔机方向舵控制器的材料。
- The order of adding the vectors is unimportant. 矢量相加的次序是无关紧要的。
- Why? One difficulty is to construct the agreements. 为什么呢?困难之一是起草这些协议。
- Construct the cardioid and its evolute. 建构心脏线及其渐屈线。
- Retrovirus is one of the vectors in common use. 逆转录病毒是最为常用的载体之一。
- Unable to construct the print dialog. 无法构造打印对话框。
- Construct the Nephroid and its evolute. [肾脏线及缩闭线].
- Parameter that linearly interpolates between the vectors. 在这些向量之间执行线性内插使用的参数。
- The Vector Product node computes the Dot Product of two vectors. 矢量积节点计算两个向量的数量积。
- The vector Z is known as the state vector of the system. 向量Z称为系统的状态向量。
- They constructed the bridge in a year. 他们花一年时间建起了那座桥。
