It was cloned into the vector of pET-28a to construct the recombinant plasmid pETLG1-3.The recombinant E. coli BL21(DE3)/pETLG1-3 was induced by IPTG.

• 用IPTG诱导重组大肠杆菌BL21(DE3)/pETLG1-3表达,SDS-PAGE分析表明在相对分子质量60000处有特异性重组蛋白表达条带,并经Western印迹进一步鉴定。