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- During the bovine oocyte maturation, this research examined the possibility of serum-free maturation which 10%FBS was replaced with 0.3%PVP. 在牛卵母细胞体外成熟培养过程中，本研究在同一种成熟培养液中，分别添加用0.;3%25PVP和10%25FBS，检验PVP代血清培养是否可行。
- The change of in vitro bovine oocyte in ultrastructure and molecular,the condition of in vitro culture,and their influence factors were discussed in this article. 本文主要对牛卵母细胞体外成熟时超微结构和分子水平的变化、受精卵体外培养的条件及其影响因素作初步探讨。
- The bovine oocyte in vitro maturation was accelerated by adding 10% bovine follicular fluid (bFF) tomaturation medium, but was restrain if adding bFF with concentration of 20% or 30% to maturationmedium. 5成熟培养液中添加10%25的牛卵泡液(bFF)对牛卵母细胞的体外成熟有促进作用,但高浓度的bFF(20%25,30%25)则抑制牛卵母细胞的体外成熟。
- The bovine oocyte in vitro maturation was accelerated by adding 10% fresh bovine follicular fluid (bFF) to maturation medium, but was inhibited if adding bFF with concentration of 20% or 30% to maturation medium. 成熟培养基中添加10%25的新鲜牛卵泡液(bFF)对牛卵母细胞的体外成熟有促进作用，但高浓度的bFF(20%25、30%25)则抑制牛卵母细胞的体外成熟。
- The effect of follicular fluid (>10mm) on bovine oocyte maturation in vitro was evaluated by addition of different concentration (0, 10, 30 and 50%, respectively) of bFF to the maturation medium. 为了确定卵泡液(直径大于10mm)对卵母细胞成熟的影响,共收集1759枚卵母细胞进行试验。
- bovine oocyte maturation in vitro 卵母细胞体外成熟
- Keywords Bovine oocyte In vitro maturation In vitro culture; 关键词牛卵母细胞;体外成熟;体外培养;
- Effects of different sera, hormones and big follicular fluid (BFF) on the maturation of bovine oocytes were investigated. 本研究比较了不同类型血清、不同来源的激素以及大卵泡液对牛卵母细胞体外成熟的影响。
- This study was undertaken to examine the effect of bovine follicular fluid (BFF) on the in vitro maturation of bovine oocytes. 本研究系统探讨了牛卵泡液（BFF）对牛卵母细胞体外成熟的影响。
- In this paper how the Uracil, ITS, and ATP affect the in vitro maturation (IVM) and viability of bovine oocytes was investigated. 摘要研究了尿嘧啶、三价因子和三磷酸腺苷二钠盐(ATP)对牛卵母细胞体外成熟及后期发育的影响。
- These results indicate that bovine oocytes can be activated by ID,IDC and EDC effectively,whereas oocytes cannot be stimulated effectively by 10 mmol/L Sr 2+ for 10 h. 结果表明 ,在 10 mm ol/ L Sr2 +内培养 10 h不能有效地激活牛卵母细胞 ,Ionom ycin+6 - DMAP± CCB以及乙醇 +6 -DMAP+CCB结合使用可以有效地激活牛卵母细胞。
- O\'Doherty Em, Wade M G, Hill J L, et al. Effects of culturing bovine oocytes either singly or groups on development to blastocysts. Therio,1997, 48:161-169. 文国艺卢克焕.;两种方法对牛体外受精及体外发育的影响[J]
- The addition of hormones(2. 5 X 103 in/L HCG, 50 in/L prolatin, 0. 05 mg/L insulin, testosterone and estradiol) to the maturation medium did not affect the positive effect of BFF on the in vitro maturation of bovine oocytes. 在成熟培养液中加入激素（2.;5×103iu/L促性腺激素（HCG），50iu/L促乳素，0
- These results indicate that BFF has a significantly inhibitory effect on the in vitro fertilization of bovine oocytes, but the thecal cell conditioned medium can improve the fertilization quality of bovine oocytes. 这些结果表明：BFF对牛卵母细胞的体外受精具抑制作用，而内膜细胞的条件液则具促进效应。
- A cow or other domestic bovine animal. 家牛牛或其它家养的属于牛属的那种动物
- Experiments were conducted to study the effects of follicle size and bovine follicular fluid (BFF) on developmental competence of bovine oocytes derived from abattoir ovaries following in vitro maturation, fertilization and culture. 以屠宰场牛卵巢为材料,研究了卵泡大小和卵泡液对牛卵母细胞体外成熟、受精和培养后发育潜力的影响。
- Different Hereford, Angus and Holstein bull semen were used for in vitro fertilization of bovine oocytes, the purpose was to observe the effects of different bull semen on in vitro fertilization. 分别利用不同个体的海伏特、安格斯和荷斯坦种公牛精液进行体外受精;观察了不同品种不同个体种公牛精液的体外受精效果.
- THE IDENTIFICATION OF CYTOPLASMIC MATURATION IN BOVINE OOCYTE WITH CORTICAL GRANULES FLUORESCENT STAINING 牛卵母细胞皮层颗粒荧光染色法质成熟鉴定
- Effect of Bovine Follicular Fluid on Maturation Rate of Bovine Oocyte Nucleus in Vitro 不同浓度的牛卵泡液对牛卵母细胞体外核成熟率的影响
- The oocyte is effectively a tetraploid cell. 卵母细胞是有效的四信体细胞。
