The addition of hormones(2. 5 X 103 in/L HCG, 50 in/L prolatin, 0. 05 mg/L insulin, testosterone and estradiol) to the maturation medium did not affect the positive effect of BFF on the in vitro maturation of bovine oocytes.

• 在成熟培养液中加入激素（2.；5×103iu/L促性腺激素（HCG），50iu/L促乳素，0