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- CONSTRUCTION OF NOS/HuIFNa CHIMERIC GENE AND ITS FUSION WITH Ti PLASMID VECTOR NOS/HuIFNa嵌合基因的構建及其與Ti質粒載體的拼接
- Nopaline was inspected in the crown gall and this data showed that T-DNA of Ti plasmid had been transformed successfully . 栝樓冠癭組織經除菌后能在無激素的MS培養基上良好生長，併合成冠癭鹼，表明Ti質粒轉化成功。
- Finally, the linear pBLI plasmid vector coated with liposomere agent,was transferred into the bFF to validate pBLI. 最後,以線性化的乳腺特異表達載體pBLI為外源基因,採用脂質體介導法轉染牛胎兒成纖維細胞。
- The researchers then used a plasmid vector to transiently increase levels of oct4 in hESC. 科學家們利用基因抑制技術（knockdown）來降低oct4基因的表達，這是一種對老鼠和人類中胚胎幹細胞進行自我更新非常關鍵的基因。
- By identifying the opines synthesized in the gall on tomato seedling incited by pathogenic strains, it was found that strains from peach, apple, hop beet and Chinese White poplar harboured nopaline type Ti plasmid. 對致病菌株的質粒類型的研究結果表明:桃樹、啤酒花和甜菜菌株基本上都是胭脂鹼(Nopaline)型質粒、葡萄菌株基本上是章魚鹼(Octopine)型質拉。
- In addition, a set of expression plasmid vectors, PMS-31b. 同時構建一組質粒表達載體PMS-31b。
- We applied real-time image and fluorescence recovery after photobleaching (FRAP) to examine the nuclear pore permeability of 27 kDa EGFP in human lung cancer cells (ASTC-a-1) transfected with plasmid vector (pEGFP-C1). 本實驗利用熒光漂白后恢復(FRAP,fluorescencerecoveryafterphotobleaching)法觀測人肺腺癌腫瘤細胞(ASTC-a-1)中表達的27kDaEGFP在核質間的被動擴散,並以激光共焦顯微鏡進行實時成像。
- The recombinant Herpesviruses of Turkey transfer plasmid vector pT-C-gB-IL was cotransfected with Herpesviruses of Turkey at CEF by calcium phosphate-DNA coprecipitation method and the target fragment was inserted into TK domain of HVT. 確定轉染時親本毒的用量,用磷酸鈣共沉澱法將HVT轉移質粒載體pT-C-gB-IL與HVT在CEF細胞上進行同源重組,將目的基因導入HVT TK區。
- METHODS: Bcl-XL siRNA and negative siRNA plasmid vector were stably transfected into A549/DDP cells. Drug sensitivity of the cells to cisplatin(DDP) was analyzed with MTT and flow cytometry. 方法:將Bcl-XLsiRNA質粒載體轉染至A549/DDP細胞,MTT、流式細胞儀檢測細胞藥物敏感性的改變;
- The human colon cancer LoVo cells were transfected by Lipofectamine 2000 with plasmid vector, and then selected for 4 weeks by G418. The expression of cerb-B1 mRNA was assessed by real time PCR and the protein of EGFR was assessed by Western blot method. 應用脂質體Lipofectamine2000轉染人結腸癌LoVo細胞,轉染成功后以G418篩選4周,實時熒光定量RT-PCR(RealTimeRT-PCR)檢測cerb-B1基因的mRNA表達,Western-blot檢測EGFR蛋白的表達。
- tumor inducing plasmid(Ti plasmid) 根瘤誘導質粒, Ti質粒[根瘤農桿菌中能誘導植物產生冠癭瘤的質粒]
- The Situation of Plasmid Vector in Gene Therapy 以質粒為載體的基因治療現狀
- Plasmid Vector Suitable for Gene Therapy or Immunization 一種基因治療和基因免疫表達載體的構建
- CONSTRUCTION OF SOIL METEGENOMIC LIBRARY WITH PLASMID VECTOR 以質粒載體構建土壤宏基因組文庫的研究
- Cloning and Sequencing of the VirD 1 Protein Gene Situating in Virus Region of Agribacterium Tumefaciens'Ti Plasmid 根癌土壤農桿菌Ti質粒毒性區VirD1基因的克隆及序列分析
- Human lung cancer cells (ASTC a 1) were infected with EGFP plasmid vectors, and incubated in RPMI 1640 culture medium supplemented with 15% FCS and 800 mg/L G418. Strong fluorescence of five cell clones were observed after several generations. 以綠色熒光蛋白 (GFP)作為標記基因轉入人類肺癌細胞系 (ASTC a 1) ;經 80 0mg/LG4 18篩選 ;獲得5株高表達細胞系 .
- In this paper, the research in optimization design for DNA vaccine was reviewed, including choice of antigen gene and immune adjuvant, optimization of plasmid vectors and inoculation methods. 許多試圖增強和調節質粒DNA疫苗的免疫原性和效率的方案都在研究之中。
- The Construction of Shuttle Plasmid Vector of Streptomyces and E. colt 鏈黴菌和大腸桿菌穿梭質粒載體的構建
- ti plasmid ti 質粒
- Did't you know he has gone ti London? 難道你不知道提已經去倫敦了嗎?
