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- 目的:探討考馬斯亮藍G250染色法在人精子畸形率、體完整率和頂體反應檢測中的應用。Objectives: To evaluate the application of Coomassie brilliant blue( CBB) G250 staining for the detection of human sperm deformity rate, rate of intact acrosome and acrosome reaction.
- 方法用Hitrap Blue層析柱去除血清中白蛋白,用U9對血清樣品進行變性處理,採用改進后的方法(0.1%甲醇,0.5%三氯乙酸,0.1%考馬斯亮藍G250)進行染色。Methods The human serum albumin was removed by Hitrap Blue column,and serum was dealed with U9.Then gel was stained by the improved method(0.1%25 carbinol,0.5%25 TCA,0.1%25Coomassie briliant blue G250).
- 考馬斯亮藍G250Coomassie Brilliant Blue G250
- 鹽酸培他啶與考馬斯亮藍G250相互作用的反應體系;coomassie brilliant blue G250 - betahistine hydrochlonide sodium chloride system.
- 考馬斯亮藍G250光度法測定水樣中陽離子表面活性劑Spectrophotometric Determination of Cationic Surfactants in Water with Coomassie Brilliant Blue G250
- 測定亞硝酸根的新指示反應研究-氯酸鉀-考馬斯亮藍G體系Study on a Indicating Reaction for the Determination of Nitrite With Potassium Chlorate-Coomassie Brilliant Blue G System
- 考馬斯亮藍Gcoomassie brilliant blue G
- 考馬斯亮藍G-250Coomassie brillant blue G - 250
- 並和考馬斯亮藍R250染色方法比較。The results were compared with traditional method(Coomassie Brilliant Blue R250).
- 考馬斯酸性藍coomassie acid blue
- 考馬斯堅牢黑coomassie fast black
- 蒽酮反應、考瑪斯亮藍、Elson Morgan反應表明 ,絮凝劑MBFTRJ2 1為粘多糖類高分子絮凝劑 ;MBFTRJ21 is mainly composed of polysaccharide, which is determined by Anthrone reaction, Coomassie blue reaction and Elson-Morgan method.
- 考馬斯海軍藍coomassie navy blue
- 考馬斯亮藍染色液coomassie blueStaining Solution
- 染色:行銀染和考馬斯亮藍染色;Staining: with silver and coomassie brilliant blue staining.
- 考馬斯亮藍微盤比色法測定蛋白質含量Protein quantification with coomassie bulliant blue microplate-colormetric
- 並且方法選擇性好,用於尿液及人血清樣品中總蛋白的測定,所得結果與考馬斯亮藍法基本一致.It is used for the determination of total protein in urine and human serum samples, the rusult obtained by this method agreeds well with those obtained by Coomassie brillant blue G?250 method.
- SDS-PAGE採用BaCl_2和I_2對mPEG染色比考馬斯亮藍染色水蛭素更為靈敏,特異性也很高。In addition, the staining of mPEG with BaCla and la is more specific and sensitive than the Coomassie Brilliant Blue staining.
- 研究了考馬斯亮藍與蛋白質的結合反應。The binding reaction of the dye coomassie brilliant blue with proteins have been studied.
- 粗毛栓菌產漆酶對考馬斯亮藍的脫色降解Decolorization and degradation of coomassie brilliant blue G-250 by laccase from Trametes gallic