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- 目的:探讨考马斯亮蓝G250染色法在人精子畸形率、体完整率和顶体反应检测中的应用。Objectives: To evaluate the application of Coomassie brilliant blue( CBB) G250 staining for the detection of human sperm deformity rate, rate of intact acrosome and acrosome reaction.
- 方法用Hitrap Blue层析柱去除血清中白蛋白,用U9对血清样品进行变性处理,采用改进后的方法(0.1%甲醇,0.5%三氯乙酸,0.1%考马斯亮蓝G250)进行染色。Methods The human serum albumin was removed by Hitrap Blue column,and serum was dealed with U9.Then gel was stained by the improved method(0.1%25 carbinol,0.5%25 TCA,0.1%25Coomassie briliant blue G250).
- 考马斯亮蓝G250Coomassie Brilliant Blue G250
- 盐酸培他啶与考马斯亮蓝G250相互作用的反应体系;coomassie brilliant blue G250 - betahistine hydrochlonide sodium chloride system.
- 考马斯亮蓝G250光度法测定水样中阳离子表面活性剂Spectrophotometric Determination of Cationic Surfactants in Water with Coomassie Brilliant Blue G250
- 测定亚硝酸根的新指示反应研究-氯酸钾-考马斯亮蓝G体系Study on a Indicating Reaction for the Determination of Nitrite With Potassium Chlorate-Coomassie Brilliant Blue G System
- 考马斯亮蓝Gcoomassie brilliant blue G
- 考马斯亮蓝G-250Coomassie brillant blue G - 250
- 并和考马斯亮蓝R250染色方法比较。The results were compared with traditional method(Coomassie Brilliant Blue R250).
- 考马斯酸性蓝coomassie acid blue
- 考马斯坚牢黑coomassie fast black
- 蒽酮反应、考玛斯亮蓝、Elson Morgan反应表明 ,絮凝剂MBFTRJ2 1为粘多糖类高分子絮凝剂 ;MBFTRJ21 is mainly composed of polysaccharide, which is determined by Anthrone reaction, Coomassie blue reaction and Elson-Morgan method.
- 考马斯海军蓝coomassie navy blue
- 考马斯亮蓝染色液coomassie blueStaining Solution
- 染色:行银染和考马斯亮蓝染色;Staining: with silver and coomassie brilliant blue staining.
- 考马斯亮蓝微盘比色法测定蛋白质含量Protein quantification with coomassie bulliant blue microplate-colormetric
- 并且方法选择性好,用于尿液及人血清样品中总蛋白的测定,所得结果与考马斯亮蓝法基本一致.It is used for the determination of total protein in urine and human serum samples, the rusult obtained by this method agreeds well with those obtained by Coomassie brillant blue G?250 method.
- SDS-PAGE采用BaCl_2和I_2对mPEG染色比考马斯亮蓝染色水蛭素更为灵敏,特异性也很高。In addition, the staining of mPEG with BaCla and la is more specific and sensitive than the Coomassie Brilliant Blue staining.
- 研究了考马斯亮蓝与蛋白质的结合反应。The binding reaction of the dye coomassie brilliant blue with proteins have been studied.
- 粗毛栓菌产漆酶对考马斯亮蓝的脱色降解Decolorization and degradation of coomassie brilliant blue G-250 by laccase from Trametes gallic