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- 结果 :Western印迹法证实APP18 81、APP18 65和APP18 58片段可与GM1分子结合 ,而APP18 51片段不与GM1分子相互作用。Immunoprecipitation Western blotting was used to identify the bindin g specialities between different gangliosides and APP 18-81 . The synthetic peptid es spanning residues 52-81 of APP blocked GM1 binding to APP 18-81 i n order to confirm the specific GM1-binding region within APP-NTFs.
- 法law
- Evans蓝法测定大鼠肺毛细血管通透性、逆转录 聚合酶链式反应 (RT PCR)法测定肺NOSmRNA表达、Western印迹法测定肺组织NOS表达 ,同时进行小肠组织学检查以评价肠损伤程度。Pulmonary microvascular permeability was evaluated by Evans blue dye concentration. Intestinal injury was assessed by histological method. The expression of pulmonary NOS (iNOS and eNOS) and its?
- 无法unable
- Western印迹Western blotting
- 用法usage
- 乙醇固定对肿瘤细胞的蛋白质提取及Western印迹的影响Effect of Ethanol Fixation on Protein Extraction and Western Blotting in Tumor Cells
- 说法statement
- 输入法input method
- Western印迹检测细胞周期蛋白D1、A和B1表达的变化。Effects on the protein expression of cyclin A,B_1 and D_1 were assessed by Western Blot.
- 除法division
- 分析法analytical method
- Western印迹试验标明,重组病毒CAV2-CGS能够表达狂犬病毒糖蛋白。2000, 8 days later, the recombinant CAV-2-CGS virus was obtained, it can express rabies strain SRV9 glycoprotein improved by Western blotting assay.
- 商法commercial law
- DNA印迹法Southern blotting
- RNA印迹法Northern blotting
- 印迹法blotting
- 免疫组化检测肾皮质磷酸化CREB1(PCREB1)及增殖细胞核抗原(PCNA)的表达特征,Western印迹及RTPCR检测肾皮质CREB1磷酸化(活性)及mRNA的变化。Upro,ucr in urine and Glu, Scr, BUN in serum were determined. Immunohistochemistry and computer image-pattern analysis system were used to analyze expression of PCNA and p-CREB_1.Western blot were performed to valuate the expression of p-CREB_1 with their specific corresponding antibodies.
- DNA印迹法Southern blotting
- 用IPTG诱导重组大肠杆菌BL21(DE3)/pETLG1-3表达,SDS-PAGE分析表明在相对分子质量60000处有特异性重组蛋白表达条带,并经Western印迹进一步鉴定。It was cloned into the vector of pET-28a to construct the recombinant plasmid pETLG1-3.The recombinant E. coli BL21(DE3)/pETLG1-3 was induced by IPTG.