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- Southern blot analysis showed that Oncidium has at least 4 copies of EIN2 in its genome. 南方墨点分析显示EIN2至少有四个拷贝分布于基因体组内。
- Rearrangement and amplificarion of erbB,sis,myc and fos in brain tumors are studied with DNA dot blot and Southern blot analysis. 本文用 DNA 斑点杂交法和 Southen 印迹法对脑瘤中 erbB、sis、myc 和 fos 这四种癌基因的扩增和重排进行了研究。
- PCR assay and Southern blot analysis showed that CMO gene was integrated successfully into the genome of transformed plant. PCR及Southern检测证明CMO基因已整合进抗性植株基因组;
- Southern blot analysis and PCR of target segment in the genome of tobacco showed that all of the plants generated were the transgenic plants. 转基因植株总DNA PCR和Southern blot分析表明目的基因已经整合到植物基因组中,但转基因的拷贝数与抗病性无明显的相关性。
- DNA dot blot analysis showed that 73. 4% of resistant plants contained GNA and hpt genes. Southern blot analysis confirmed the integration of GNA gene in the genome of transgenic rice. DNA点杂交检测表明73.;4%25的抗性植株同时含有GNA基因和hpt基因;Southern杂交分析进一步证实了GNA基因在转基因水稻基因组中的整合
- Organogenic transgenic calli and transgenic regenerated plantlets were produced on selection medium containing 15mg/L kanamycin, and confirmed by GUS histochemical staining, polymerase chain reaction (PCR), and southern blot analysis. 器官发生的转基因愈伤组织和转基因的再生植株已经获得 ,并经GUS组织化学染色、聚合酶链式反应和Southern杂交分析证实。
- Dot blot and southern blot analyses suggested that gfp gene is integrated into the genome of transgenic plants of Nicotiana tabacum, Pentunia genome. 通过对有绿色荧光的烟草、矮牵牛进行dot blot、southern blot分析，都获得阳性杂交结果，证实gfp基因已整合到植物基因组中并与绿色荧光观察结果一致。
- Southern blotting analysis of genomic DNA indicated that enolase was likely to be a low-copy gene in the oilseed rape genome. Southern杂交结果显示烯醇酶以低拷贝形式在油菜基因组中存在.
- The expression vector was linearized and transformed protoplasts of Aspergillus niger strain An1. Hygromycin-resistant transformants were generated and the integration of xylanase gene was confirmed by genomic Southern blotting analysis. 将该表达载体线性化后转化果胶酶产生菌黑曲霉菌株An1的原生质体。 对抗潮霉素黑曲霉转化子进行基因组Southern杂交分析结果表明,木聚糖酶基因已整合到受体基因组中。
- Using Southern Blot Analysis to Determine the Integration of Transduced Genes in the Genomic DNA of Host Cells 应用Southern印迹杂交法检测转移基因在宿主细胞中的整合
- fps gene probe was signed with DIG High Prime, PCR- Southern blot analysis with PCR production of fps gene, proved fps gene was expressed in molecule level. 利用地高辛标记fps基因探针，对fps基因PCR产物进行PCR-Southern检测，并且检测到了目的基因片段的印迹，证明fps基因在发状根基因组分子水平得到了表达；
- Southern blot technique developed to identify DNA fragments. 印迹杂交被用来发现DNA片段。
- This CTB gene product was also verified by Western blot analysis. Western blotting结果确认了该条带为CTB基因的产物。
- The results of western blot analysis were in accordance with those of 2-DE. 对部分差异蛋白采用Western-blot方法在蛋白水平的验证结果与2-DE结果基本相符。
- Regenerated plants with kanamynic resistantance were obtained. PCR , PCR-Southern blot analysis , southern dot blot analysis of lettuce DNA confirmed that adw gene had been integrated into the plant genome. 细胞核载体PB-adw、PBG-adw均采用农杆菌介导法将adw导入莴苣，细胞核转化获得了生长良好的抗性莴苣植株，经PCR、PCR-Southern、southern斑点杂交分析证实，adw基因已整合到莴苣基因组中。
- We detected 213 specimens from 173 people using probe of pBR-322 by Dot and Southern blot hybridization. 我们用常见的克隆载体pBR322作探针,用核酸点杂交和Southern吸印方法,检测了来自173人的213例样品。
- Southern Blot with positive plants of PCR showed that CMO and BADH have been integrated into the rice genome. 对随机抽取的PCR检测双阳性植株进行Southern blotting杂交检测分析，表明CMO和BADH基因均已整合到转基因水稻的基因组中。
- Transgenic potato plants 1M, 2M, 3M, 5M, 10M, llM provided by laboratory were analyzed by Southern blot and Northern blot. ELISA检测转基因马铃薯病毒抗性数据显示，温室条件下，3个转基因马铃薯株系(2M、3M、11M)具有病毒抗性，3个株系(1M、5M、10M)感病；
- RT-PCR, Western blot analysis and FACS were used to detect the expression of P2X7 receptor in these clones. 荧光分光光度计检测P2X7受体介导的胞内钙离子浓度变化。
- Using morphology observation, the RT-PCR and Western blot analysis to investigate and analysis the sub-type of CnA. 通过病理形态学检查、RT-PCR实验测定及Western blot分析确认CnA亚型。
