Regenerated plants with kanamynic resistantance were obtained. PCR , PCR-Southern blot analysis , southern dot blot analysis of lettuce DNA confirmed that adw gene had been integrated into the plant genome.

• 细胞核载体PB-adw、PBG-adw均采用农杆菌介导法将adw导入莴苣，细胞核转化获得了生长良好的抗性莴苣植株，经PCR、PCR-Southern、southern斑点杂交分析证实，adw基因已整合到莴苣基因组中。