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Plant expression constructs 植物表达载体
The coat protein gene of GPV, a Chinese serotype isolate of BYDV, was used to construct three plant expression vectors, pPPI 2, pPPI 3 and pPPI 5, which contain Act promoter or Emu promoter respectively. 以我国特有的大麦黄矮病毒 GPV株系的外壳蛋白 ( CP)基因为材料 ,设计合成了分别含有 Act启动子或 Emu启动子的植物表达载体 p PPI2、p PPI3和 p PPI5。
In this study, we combine ocs activator elements with mas promoter, then fuse the chimeric promoters on GUS gene to construct plant expression vectors. We also demonstrate by comprison to the 35S promoter. 融合启动子与GUS报告基因融合构建相应的植物表达载体，并以含CaMV35S启动子的表达载体pBI121作对照转化烟草NC89，利用荧光分光光度计法定量测定各转基因烟草植株的GUS基因表达活性。
And then the CED-9 gene was inserted into plant expression vector pBI121 under the control of CaMV 35S promoter. 在此基础上再构建重组表达载体pBI-ced9，将CED-9基因置于CaMV35S启动子控制之下。
The BADH and CMO ORF were obtained and the plant expression vector pCU1303-BADH, pCU1303-CMO were constructed. 设计带特定酶切位点的特异引物,分别克隆了麦冬BADH和CMO的编码区片段,构建了植物表达载体pCU1303-BADH和pCU1303-CMO。
A doublegene plant expression vector,pC35SC35SB1303 were reconstructed based on PC1303 plasmid by CMO and BADH which were both driven by 35S promoter. 以pC1303质粒为基础,构建了均由35S启动子驱动的CMO基因和BADH基因的植物双基因表达载体pC35SC35SB1303。
Through the gene gun to transfer the AWTE CTB plant expression vector pBVG ny2 which containing NPTII and GUS gene into the soybean tissue. 把 AWTE- CTB融合基因构建到植物表达载体 p BVG- ny2上 ,通过基因枪导入法 ,转化大豆幼胚分生组织。
Important developments show that plant expression of a dsRNA targeting a nematode gene can successfully induce silencing in parasitizing nematodes. 研究进展表明，植物双链RNA表达靶定的线虫基因能够成功地诱导线虫基因沉默。
The fused gene STI-STII-LTB was cloned into plant expression vector pBI121-MARs to replace GUS gene, another plant expression vector was constructed without MAR sequence. 将融合基因STI-STII-LTB构建到带有核基质结合区序列(MARs)的植物表达载体pBI121-MARs中GUS基因的位置。
Plant expression vector p35EZ was constructed to transform the explants of Cucumis sativus L. by Agrobacterium tumefaciens (Smith et Townsend) Conn Ti plasmid_mediated method. 将该基因克隆在pBI1 2 1的 35S启动子和Nos终止子之间 ;得到植物表达载体p35EZ。通过根癌农杆菌 (Agrobacteriumtumefaciens (SmithetTownsend)Conn)介导的方法转化黄瓜 (CucumissativusL .;)
Plant expression vector pPCHf,pPCCM were constructed through replaced the GUS in pPCHS by flower color gene Hf2 and floral-specific transcription factor CMB2 respectively. 将花特异表达启动子PCHS分别与花色基因Hf2、花特异转录因子CMB2构建植物表达载体pPCHf、pPCCM,将组成型启动子CaMV35S与花特异转录因子CMB2构建植物表达载体pCM。
A recursive table is a common table expression constructed as a result of a WITH clause in a query. 递归表是作为查询中的WITH子句的结果构建的公用表表达式。
The plant expression vectors with and without MARs were transferred into tobaccos (Nicotiana tabacum L.) via Agrobacterium-mediated transformation procedure. 将此载体与不包含MARs序列的植物表达载体经根癌土壤杆菌(Agrobacterium tumefaciens (Smithet Townsend) Conn)介导转化烟草(Nico tianatabacum L.;)。
Based on the safety coefficient expression constructed in the stability calculation process, the value in geotechnique design is illustrated. 以此为基础，通过剩余推力法和Sarma两种方法评价了其边坡稳定性，并以稳定性计算中构建的安全系数为基础，阐明了其在岩土工程设计中的意义。
The plant was growing at an angle. 植物呈一定角度生长。
The plant expression vector pC-pPro-2-TCS was constructed with TCS structure gene integrated into downstream of PAL inducible promoter carried by pPPG expression vector. TCS gene was controled by linked promoter-PAL. pC-pPro-2-TCS是在pPPG表达载体的PAL诱导型启动子下游嵌合入TCS基因,TCS基因在串联启动子PAL控制下;
Obtaining of MCEMA(modified CEMA) and plant defensin AFP geneMCEMA (141bp) was amplified with pUCSPCEMA plasmid as template and P5, P4 as primers, and plant expression vector pEMCEMA was constructed. MCEMA和植物防御素基因的PCR合成以经测序验证的SPCEMA为模板，用P_5和P_4扩增得到了全长141bp的MCEMA，并构建了植物表达载体pEMCEMA。
The CMO ORF was cloned and the plant expression vector pBI121-CMO was constructed. The resultant plasmids pBI121-CMO was transferred into Agrobacterium tumefaciens (GV3101) by the liquid nitrogen freezing thaw method. 克隆了CMO基因的编码区,构建了其植物表达载体pBI121-CMO,冻融法将质粒导入根癌农杆菌(Agrobacterium tumefaciens)GV3101,获得工程菌GV3101-pBI121-CMO。
In this study, with the help of the intermidiate vector pJIT163,2-5A gene and Rnase L gene were cloned into the binary plant expression vector pBINplus to obtain the plant transformation/expression vector pBIN2-5A and pBINRL. 本文利用2-5A基因和RNase L基因,通过中间载体pJIT163将2-5A基因和RNase L基因克隆到双元植物表达载体pBINplus上,分别构建了2-5A基因的植物表达载体pBIN2-5A和RNase L基因的植物表达载体pBINRL。
The MP fragments were then fused with constitutive expression promoter CaMV 355 and pathogen - inducible promoters CHS, Pill and BG, respectively, which led to the consmiction of plant expression vectors p355-30K, pPiII-30K, pPiII-30K and pBG-30K. MP片段分别与组成型启动子CaMV35S和病原特异诱导型启动子CHS、PiII和BG体外重组，构建成植物表达载体P35S－30K，pCHS－30K，pPiII－30K和pBG－30K。通过农杆菌LBA4404介导，分别转化含Tin－22抗性基因的番茄品种Geneva80。