Obtaining of MCEMA(modified CEMA) and plant defensin AFP geneMCEMA (141bp) was amplified with pUCSPCEMA plasmid as template and P5, P4 as primers, and plant expression vector pEMCEMA was constructed.

• MCEMA和植物防御素基因的PCR合成 以经测序验证的SPCEMA为模板，用P_5和P_4扩增得到了全长141bp的MCEMA，并构建了植物表达载体pEMCEMA。