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- PCR产物克隆测序Sequence analysis with clone of PCR products
- 对Sry-PCR产物克隆测序,得到185bp的Sry基因部分核苷酸序列。The partial nucleotide sequence(185 bp)of the Roe deer Sry gene was determined by cloning the PCR product.
- 方法 :用聚合酶链反应 (PCR)扩增HPV58L1完整编码区基因 ,将PCR扩增产物克隆至 pUC1 9质粒中并测序。Methods:The full length L1 coden region of HPV58 was amplified by PCR,and cloned into pUC19,sequenced.
- PCR产物直接测序Sequence analysis with PCR products
- 通过BP反应将包含有attB接头的PCR产物克隆到含有attP的donor载体上以产生Entry克隆,通过LR反应将已经重组入Entry载体的DREB1A基因再克隆到pH2GW7双元载体。By the BP recombination reaction,the PCR product containing attB was transfered to an attP-containing donor vector to create an entry clone. Finally,DREB1A gene was shutted into pH2GW7 vector by LR recombination reaction.
- 克隆测序cloning and sequencing
- 利用PCR技术从猪细小病毒的RFDNA模板中扩增了含有VP2 全基因的 2 0kb的基因片段 ,将PCR产物克隆至 pMD18 T载体 ,利用双脱氧末端终止法测定了VP2 全基因的核苷酸序列。The completed VP 2 gene was amplified from PPV RFDNA by PCR method. Then the products were cloned into pMD18-T vector and the sequence was determined.
- 克隆测序技术在小鼠心肌炎实验中的应用Application of the Colone and DNA Sequence Test in Myocarditis Mice Model
- coliO_(157):H_7EDL933株的rfbE基因和fliC基因,设计了两对引物,分别对两个O_(157):H_7菌株的rfbE基因和一个菌株的fliC基因进行PCR,并将PCR产物克隆于pMD18-T载体质粒。coli O_(157):H_7 , two primers were designed according to the sequence of rfbE gene and fliC gene of E. coli O_(157):H_7 EDL933 strain. The part fragment of rfbE gene of two E.
- 埃及伊蚊抗凝血因子Xa基因片段的克隆测序Part Gene Sequence of Factor Xa-directed Anticoagulant from the Vector Mosquito Aedes aegypti in China
- 可直接克隆PCR产物的克隆载体的构建Construction of A New Vector for Direct Cloning of PCR Products
- PCR测序PCR sequencing
- 产物测序结果The results of sequencing of PRRSV PCR amplified products from Inner Mongolia sequenced
- 方法采用PCR法从结核杆菌H37Rv株扩增Ag85A基因,将PCR扩增产物克隆于2型腺相关病毒(AAV-2)表达质粒pSNAV中,构建重组质粒pSNAV-Ag85A;Methods Ag85A gene was amplified by polymerase chain reaction (PCR) from the genome of Mycobacterium tuberculosis H37Rv strain.
- 麦洼牦牛肥胖基因部分片段的克隆测序研究Cloning, Sequencing Research on Partial Fragment of Obese Gene of Bos grunniens
- PCR测序法PCR sequencing
- 利用RT-PCR方法扩增了肉鸡HSP70 mRNA,将其扩增产物克隆到PGEM-T Easy载体和原核表达载体pET-28a(+)上,进一步转化至大肠杆菌BL-21中,用IPTG诱导表达重组肉鸡HSP70。The mRNA of broiler HSP70 gene was amplified by RT-PCR and the production was cloned into the PGEM-T Easy vector and the expression vector pET-28a (+) . The recombinant expression vector was transformed into E. coli BL-21 and induced by IPTG to express.
- PCR测序(法)PCR sequencing
- 新疆褐牛weaver位点部分序列的克隆测序及分析Partial Cloning and Analysing of Weaver Gene in Xinjiang Brown Cattle
