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- The embryo production in vitro include oocytes in vitro maturation (IVM), in vitro fertilization (1VF), parthenogenetic activation (PA), in vitro culture (IVC) and sex identification. 胚胎体外生产包括卵母细胞体外成熟、体外受精、孤雌激活、胚胎体外培养和性别鉴定等主要环节,本研究针对影响各个环节的关键因素进行研究,旨在不断完善胚胎体外生产技术。
- In vitro Maturation of Vitrified GV Stage Oocytes in Pig 猪GV期卵母细胞玻璃化冷冻后的体外成熟
- The bovine oocyte in vitro maturation was accelerated by adding 10% bovine follicular fluid (bFF) tomaturation medium, but was restrain if adding bFF with concentration of 20% or 30% to maturationmedium. 5成熟培养液中添加10%25的牛卵泡液(bFF)对牛卵母细胞的体外成熟有促进作用,但高浓度的bFF(20%25,30%25)则抑制牛卵母细胞的体外成熟。
- The bovine oocyte in vitro maturation was accelerated by adding 10% fresh bovine follicular fluid (bFF) to maturation medium, but was inhibited if adding bFF with concentration of 20% or 30% to maturation medium. 成熟培养基中添加10%25的新鲜牛卵泡液(bFF)对牛卵母细胞的体外成熟有促进作用,但高浓度的bFF(20%25、30%25)则抑制牛卵母细胞的体外成熟。
- Keywords Oocytes In Vitro Maturation;Intracytoplasmic Sperm Injection(ICSI);In Vitro Fertilization;Embryo Transfer(ET);Polycystic Ovary Syndrome(PCOS); 卵母细胞体外成熟;单精子卵胞浆内注射;体外受精;胚胎移植;多囊卵巢综合征;
- Oocytes In Vitro Maturation 卵母细胞体外成熟
- The Role of Cumulus Cell in Oocyte in Vitro Maturation 卵丘细胞在卵母细胞体外成熟培养过程中的作用
- Keywords Bovine oocyte In vitro maturation In vitro culture; 关键词牛卵母细胞;体外成熟;体外培养;
- Methods Rhesus monkey blastocysts were obtained after in vitro maturation of oocytes, fertilization and maturation of early embryos. 方法猕猴卵母细胞经体外成熟培养、外受精和早期胚胎体外成熟培养后,获得猕猴囊胚。
- This study was undertaken to examine the effect of bovine follicular fluid (BFF) on the in vitro maturation of bovine oocytes. 本研究系统探讨了牛卵泡液(BFF)对牛卵母细胞体外成熟的影响。
- In this paper how the Uracil, ITS, and ATP affect the in vitro maturation (IVM) and viability of bovine oocytes was investigated. 摘要研究了尿嘧啶、三价因子和三磷酸腺苷二钠盐(ATP)对牛卵母细胞体外成熟及后期发育的影响。
- Its latest relevant research and progress were summarized, including ovary oocytes collection, in vitro maturation of oocytes, sperms' in vitro capacity and fertilization. 对近年来羊卵巢卵母细胞体外受精技术的研究现状和进展进行了综述,其中包括卵巢卵母细胞的采集、卵母细胞体外成熟、精子体外获能、体外受精。
- Objective: To study the possibility of in vitro maturation, fertilization and embryo developmentas well as pregnancy of immature oocytes with polycystic ovary (PCO). 目的:探索在不用或小剂量使用促性腺激素条件下对多囊卵巢患者的未成熟卵母细胞体外培养至成熟、受精、卵裂和妊娠的可行性。
- Studies on Porcine Oocyte in Vitro Maturation (IVM) Pathenogenetic Activation and Apoptosis 猪体外成熟卵母细胞孤雌激活及凋亡观察
- The addition of hormones(2. 5 X 103 in/L HCG, 50 in/L prolatin, 0. 05 mg/L insulin, testosterone and estradiol) to the maturation medium did not affect the positive effect of BFF on the in vitro maturation of bovine oocytes. 在成熟培养液中加入激素(2.;5×103iu/L促性腺激素(HCG),50iu/L促乳素,0
- The objective of the present study was to examine the effects of porcine follicular fluid (pFF) and fetal calf serum (FCS) on in vitro maturation of porcine oocytes derived from small follicles (diameter <2 mm). 本研究探讨了猪卵泡液(pFF)和胎牛血清(FCS)对猪小腔卵泡卵母细胞体外成熟的影响。
- Experiments were conducted to study the effects of follicle size and bovine follicular fluid (BFF) on developmental competence of bovine oocytes derived from abattoir ovaries following in vitro maturation, fertilization and culture. 以屠宰场牛卵巢为材料,研究了卵泡大小和卵泡液对牛卵母细胞体外成熟、受精和培养后发育潜力的影响。
- This experiment studied the effects of ova pick-up methods, bFF,culture period and different culture systems on in vitro maturation of Nanyang cattle oocytes derived from small antral follicles. 探讨了不同采集方法、牛卵泡液(bFF)添加浓度、培养时间和不同培养体系对南阳牛小腔卵泡卵母细胞体外成熟的影响。
- Methods:Collected the cumulus-oocyte complexes at germinal vesicle stage and cryopreserved them by vitrification,then followed by in vitro maturation(IVM),in-vitro fertilization(IVF) of the oocytes and in-vitro culture of the embryos(IVC). 方法:小鼠GV期卵母细胞用OPS法玻璃化冷冻复苏后行体外成熟培养(in-vitro m aturation,IVM)或直接行IVM,所获成熟卵行体外受精(IVF)和胚胎的体外培养(IVC)。
- To use in vitro maturation(IVM) for treatment of infertile women with polycystic ovary syndrome(PCOS).[Methods] Totally 152 immature oocytes were retrieved from 23 PCOS women and cultured in vitro. 应用卵母细胞体外成熟(IVM)技术治疗多囊卵巢综合征不孕症。[方法]23例患者中的152个未成熟卵母细胞进行体外培养,成熟后行单精子卵胞浆内注射,受精后取优质胚胎移植宫腔,同时对其影响因素进行探讨。