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- Hyperthermia can significantly enhance the sensitivity to cisplatin in human multidrug-resistant lung cancer cell line A549/DDP. 热疗能提高人肺腺癌耐药细胞株A549/DDP对顺铂的敏感性,热化疗具有协同作用。
- Objective To establish a successful xenotransplantation model of a human small cell lung cancer cell line (NCI_H446) in nude mice. 目的建立成瘤稳定、潜伏期短的人小细胞肺癌NCI_H446细胞裸鼠移植瘤模型。
- Objective To construct recombinant retroviral vector expressing antisense ANG and transfect it into the human lung cancer cell line A549 to inhibit the expression of ANG. 目的 构建含反义人血管生成素的真核表达载体 ,并转染人肺癌细胞株A5 4 9,使之特异性地封闭A5 4 9细胞中血管生成素的表达 ,以达到抑制肿瘤生长的目的。
- Cole,S P,Bhardwaj G,Gerlach J H et al.Overexpression of a transporter gene in a multidrug-resistant human lung cancer cell line[J].Science,1992,258 (5088):1650. 石玉枝;刘静蕾;霍建民;等.;三氧化二砷对人肺腺癌裸鼠模型抗肿瘤作用的实验研究[J]
- Objective In order to observe the infiltration and metastasis of the tumor,establish a high-metastatic lung cancer cell line expressing enhanced green fluorescent protein(EGFP). 目的建立能稳定高表达增强型绿色荧光蛋白(EGFP)的肺癌细胞株,为研究肺癌的浸润与转移机制打下基础。
- The present study observed the effects of superoxide dismutase (SOD) and itsinhibitor, diethyldithiocarbamate (DDC), on the metastasis of Lewis lung cancer andsome biological characteristics of A_(549) lung cancer cell line. 本实验用SOD及其抑制剂DDC分别对Lewis肺癌和A_(549)肺癌细胞进行了实验研究,以观察SOD在肺癌的某些生物学特性中的作用。
- Objective To study selective killing effect of herpes simplex virus-thymidine kinase/ganciclovir(Hsv-tk/GCV) driven by human telomerase catalytic subunit (hTERT) promoter on lung cancer cell line A549 in vitro. 目的研究人端粒酶催化亚单位(human telomerase catalytic subunit,hTERT)基因启动子调控单纯疱疹胸苷激酶/更昔洛韦(herpes simplex virus-thymidine kinase/ganciclovir,Hsv-tk/GCV)治疗系统对人肺癌细胞株A549的选择体外杀伤作用。
- Method: We used confocal microscopy observed the expression of the thrombocytic immune related antigen CD42a, TSP and CD9 on high metastasis lung cancer cell line PG and low metastasis lung cancer cell line PAa. 方法:利用激光共聚焦显微镜观察高转移人巨细胞肺癌细胞PG利低转移人肺腺癌细胞PAa表达的血小板免疫相关抗原CD42a、TSP、CD9的表达情况;
- In this study, the dicistronic expression vector (pEGFP-C1) was used to be transfected into human lung cancer cell line(ASTC-a-1)and a positive clone which stably expressed GFP in high level was obtained. 利用常规转染方法将带有EGFP基因的质粒载体导入人肺腺癌肿瘤细胞 (ASTC a 1) ,并得到GFP稳定表达的细胞株。
- Methods: IRE-EGFP-esp1 plasmid was transfected into lung cancer cell line A549 via FUGENE 6 liposome, ploidy, and cell cycle were studied by flow cytometry combined with fluorescent staining,and DNA index (DI) and SPF were calculated. 方法:将IRE-EGFP-esp1质粒用Fu-GENE6脂质体转染到肺腺癌细胞A549,经G418筛选获得转染阳性的细胞系(esp1-A549)。 采用DNA荧光染色流式细胞仪分析转染后细胞DNA含量、倍性及细胞周期,计算S期细胞比率(SPF)和DNA指数(DI);
- The mRNA levels of this gene were determined in SCLC cell line SH77 ,big cell lung cancer cell line H460,lung adenocarcinoma cell line SPC A 1 and SPC A 1/CDDP using semi quantitative RT PCR assay. 应用半定量RT-PCR方法检验小细胞肺癌SH77、大细胞肺癌H460、肺腺癌SPC-A-1和SPC-A-1/cDDP等细胞株中该基因mRNA的表达。
- Conclusion: The phototoxic effects of HB-PDT on DDP-resistant lung cancer cell lines have no significant difference. 结论:HB-PDT对于耐药及非耐药细胞的光毒效应无显著差别。
- METHODS:H1299(human non-small cell lung cancer cell line)cells were co-transfected with recombinant plasmid pEF-BOS/GST-EGFR-TKD and pBabe-puro by calcium phosphate transfection. 方法:采用磷酸钙共沉淀基因转染技术,将已构建的pEF-BOS/GST-EGFR-TKD质粒DNA与pBabe-puro质粒DNA共同导入体外培养的人非小细胞肺癌细胞系H1299中。
- Objective To investigate the correlation between BRI gene expression and the metastatic potential in human non small cell lung cancer cell lines. 目的 研究BRI基因在人非小细胞肺癌细胞系中的表达 ,探讨其表达情况与肺癌转移能力的相关性。
- The experimental results showed that overexpressing the KLK8 gene in highly invasive lung cancer cell lines suppressed their invasiveness. 实验结果显示藉由转殖于具有高侵袭力肺癌细胞株中人类第八号激肽释放酶基因的过量表现,可抑制肺癌细胞的侵袭力。
- Decreased RASGRF2 expression was presented in 36% (5/14) lung cancer cell lines while aberrant methylation of RASGRF2 was present in 29% (4/14) lung cancer cell lines. RASGRF2 在 36%25 (5/14)的肺癌细胞中表达降低,而在 29%25 (4/14) 肺癌细胞中存在异常甲基化。
- human A459 lung cancer cell line 人肺腺癌细胞株A549
- human lung cancer cell line A549 A549人肺腺癌细胞
- WSW also selectively replicated in murine and human lung cancer cell lines but not in normal cells. WSW具有选择性在肿瘤细胞复制的能力,而不论在人类或小鼠正常细胞中皆无毒杀作用。
- human lung cancer cell line H460 人肺癌细胞株H460