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- Cytokine-induced kill cells (CIK) is a new kind of immunologically competent cell and have ability of strong reproductive activity. 摘要细胞因子诱导的杀伤细胞(CIK)是一种新型的免疫活性细胞,CIK增殖能力强,作用强,具有一定的免疫特性。
- An efficient bacterial transformation system was described for the preparation of competent cells, plasmid preparation, and for the storage in bacterial stocks. 描述了一种改良的高效细菌感受态的制备和感受态细胞的保藏及细菌的转化方法。
- The recombinant plasmid containing ATF-PAI2CD fused gene, pZWY-ATF-PAI2CD, was transferred to the competent cells of yeast strain GS115. After the phenotype selection, the positive clones were induced with methanol to express ATF-PAI2CD. 该质粒转化毕赤酵母菌GS115 ;用G4 18 YPD平板筛选高拷贝转化子 ;然后用甲醇诱导表达 .;工程菌用摇瓶发酵;表达产物ATF PAI2CD占培养液中总蛋白 5 0%25以上
- Methods: Recombinant plasmid pFHTNS3 was constructed by inserting HGV NS3 gene into transposing vector pFastBac HTa. pFHTNS3 plasmid was further transformed into DH10Bac competent cells. 方法 :将 HGV NS3基因片段定向克隆至转座载体 p Fast Bac HTa,转化 DH10 Bac感受态细胞 ,37℃振荡培养 4h使发生转座 ,用抗生素平皿筛选重组 bacmid。
- Then they were transformed into Escherichia coli BL21(DE3) competent cells for expression. The expressed nucleoprotein was purified using Ni2+-NTA Column under denaturing conditions. 将重组质粒转化到大肠杆菌BL21(DE3)中进行表达,表达的核蛋白再经Ni2+-NTA亲和层析纯化。
- One RING domain and two BIRs domain contained in IAP2 while one RING and one BIR domain contained in IAP3.iap2 and iap3 were ligated into expression vector pET-28a, then were transformed into BL21(DE3) competent cell respectively. 用表达载体pET28a-iap2转化宿主菌BL21(DE3)感受态细胞,挑取单菌落并接种于加卡那霉素的LB中,扩大培养后加入IPTG诱导表达,表达结果显示,含iap2基因载体的表达产物在电泳中出现一条约40kDa的表达带,与理论推测的蛋白分子量一致。
- virgin immunologically competent cells 未接触抗原性免疫活性细胞
- Electro Competent Cell Preparation Kit 电转感受态细菌制备试剂盒
- One Step Competent Cell Preparation Kit 产品简介:碧云天生产的一步法感受态细菌制备试剂盒
- Calcium Competent Cell Preparation Kit 钙转感受态细菌制备试剂盒
- immunologically competent cell(ICC) 免疫活性细胞, 免疫感受态细胞
- uncommitted immunologically competent cell 未定型的免疫活性细胞
- virgin immunologically competent cell 未接触抗原的免疫活性细胞
- He is competent enough to fill that position. 他完全胜任那个职位。
- We know you are competent in doing this job. 我们知道你可以胜任这项工作。
- I summed her up as a competent manager. 我认为她是个很能干的经理。
- committed immunologically competent cell 定型免疫活性[适格]细胞
- Mark proved to be a very competent manager. 马克证明了自己是一个很能干的经理。
- Human tissue is made up of cells. 人体的组织是由细胞构成的。
- We have to admit that he's a highly competent man. 我们必须承认他是个非常能干的人。