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Enzyme reporter gene 酶报告基因
There are three kinds of biosensors relevant to bioluminescence &chemiluminescence: immobilized enzyme sensor, immunoassay sensor and report gene sensor with specialized regulator. 基于生物及化学发光的生物传感器主要有三类 :酶固化传感器、免疫分子传感器和特异调控因子诱导的报告基因传感器。
Thus we constructed reporter vectors in which the reporter gene is luciferase. 经酶切鉴定以及测序分析确定载体构建正确。
SuperLight Luciferase Reporter Gene Assays for gene regulation and modulator screen. 荧光素酶报告基因检测试剂盒,用于基因调控筛查的。
This promoter is shown to direct the specific expression of the reporter gene,B-glucuronidase(GUS), in trichomes of Arabidopsis. 将此启动子与gus报告基因相连构建植物表达载体并转基因拟南芥，GUS组织化学定位分析表明此启动子在拟南芥叶片和茎的表皮毛细胞中特异表达。
The transient transfection of Stat 3 cDNA into COS7 cells increased Stat3 protein level and the expression of the reporter gene. Stat3cDNA和报道基因在COS7细胞中的共转染实验表明 ,外源的Stat3蛋白具有DNA结合活性 ,并可增强报道基因的表达。
Transient expression vectors were constructed by fusing the promoter fragment with gus reporter gene and nopaline terminator in different orientation. 将启动子片段分别以不同方向与gus报告基因和nos终止子融合 ,构建了瞬时表达载体。
Transient transfection of Stat3 cDNA into COS7 cells increased Stat3 protein level and also the reporter gene expression. Stat3 cDNA和报告基因在COS7细胞中的共转染实验表明，外源的Stat3蛋白具有DNA结合活性，并可增强报告基因的表达。
Reporter gene imaging is an important imaging technique in molecular imaging,which can image various biological and genetical processes. 报告基因显像技术是分子影像学中重要的显像技术,其可对多种不同的生物学和分子遗传学过程进行显像,有望更快地应用于临床。
The minor T allele significantly attenuates reporter gene expression (P<0.01) and is impaired in its capacity to form DNA-protein complexes (P<0.05). 该等位基因处如为T可使基因表达量显著下降，并损伤其形成DNA-蛋白复合体的能力。
SarA exhibiting an up-regulation on lipA and zinC, and down-regulation on atlE were demonstrated by reverse-transcription PCR and reporter gene lacZ analysis respectively. RT-PCR和lacZ报告基因的分析结果显示;在表皮葡萄球菌ATCC35984中;SarA对atlE(自溶酶基因)表达起负调控作用;而对lipA(脂肪酶基因)和zinC(膜相关锌金属蛋白酶基因)的表达则有正调控作用.
GFP reporter gene under the direction of HBV promoter(enhancer) could be expressed in tumor cells and be expressed definitely and specifically in HCC Bel7402 cell. HBV启动子调控下的GFP报告基因能够在肝癌Bel740 2细胞中表达并具有一定的专一性。
Plasmids pRecAgfp and pUvrAgfp were constructed after DNA damage-inducible promoters of recA and uvrA from Escherichia coli were fused to the reporter gene gfpmut3a operon. 将报告基因绿色荧光蛋白基因(gfpmut3a)分别置于recA和uvrA启动子调控下;构建成质粒pRecAgfp和pUvrAgfp;并转化E.
By fusing this two fragment with gus reporter gene and nopaline terminator, we get two transient expression vectors which named pCAMBIA-ACTP and pBI-ACTPS. 将该启动子两个片段分别与gus报告基因和nos终止子融合，构建成瞬时植物表达载体pCAMBIA-ACTP和pBI-ACTPS。
Recently HSV1-TK reporter gene imaging has been widely and deeply studied, the findings showed the HSV-TK reporter gene 4mnging may solve these problems. 近年来，HSV1-TK报告基因显像已进行了广泛而又深入的研究，结果显示它可解决目前存在的这些问题。
Recently HSV1-TK reporter gene imaging has been widely and deeply studied, the findings showed the HSV-TK reporter gene imaging may solve these problems. 近年来，HSV1-TK报告基因显像已进行了广泛而又深入的研究，结果显示它可解决目前存在的这些问题。
The reporter had a red-hot story. 那个记者掌握著最新消息。
By using lucifirase as reporter gene, F9 cells were transfected with a series of reporter constructs containing the subclones of the flanking sequences and their nested deletions. 利用荧光素酶为报告基因，构建了一系列含有侧翼序列亚克隆及其缺失体报告基因质粒，并转化了F9细胞。分析这些亚克隆及其缺失体对报告基因活性的影响。
Recently HSV1-TK reporter gene imaging has been widely and deeply studied,the findings showed the HSV-TK reporter gene imaging may solve these problems. 近年来,HSV1-TK报告基因显像已进行了广泛而又深入的研究,结果显示它可解决目前存在的这些问题。
By using lucifirase as reporter gene, F9 cells were transfected with a series of repoter constructs containing the subclones of the flanking sequences and their nested deletions. 利用荧光素酶为报告基因,构建了一系列含有侧翼序列亚克隆及其缺失体报告基因质粒,并转化了F9细胞。分析这些亚克隆及其缺失体对报告基因活性的影响。