By using lucifirase as reporter gene, F9 cells were transfected with a series of repoter constructs containing the subclones of the flanking sequences and their nested deletions.

• 利用荧光素酶为报告基因,构建了一系列含有侧翼序列亚克隆及其缺失体报告基因质粒,并转化了F9细胞。分析这些亚克隆及其缺失体对报告基因活性的影响。