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- The results manifested that both BRL-CM and common medium were suitable for porcine EG cells culturing and passaging when having STO feeder cells. 结果表明,以STO细胞作饲养层,使用BRL条件培养基与常规培养基在猪EG细胞分离培养、传代后均可获得细胞集落,而且使用BRL条件培养基培养产生的集落数多,细胞生长速度快。
- When cultured on degenerated feeder layers or in suspension, EG cells formed embryoid bodies (EBs) in vitro. 当EG细胞脱离饲养层悬浮培养,或在衰老的饲养层上延迟培养时,发现EG细胞或单个存在,或聚集成团,形成类似于早期胚胎的囊状胚体结构.
- MEF, STO, BEF and HEF feeders all can promote growth of bovine EG cells in vitro, but STO is the best, HEF is the last and MEF, BEF is in the middle. MEF、STO、BEF及HEF饲养层均能促进牛EG细胞体外增殖,但STO效果最好,MEF及BEF次之,HEF效果最差。
- Contributions of the WZSP EG cells to development are not detectable in these tissues: heart,lung,liver,spleen,kidney,jejunum and ovary. 结果表明:本研究中所获得嵌合体猪的皮肤、大脑、胰、甲状腺、血有外源基因嵌合,而其肺、肝、脾、肾、空肠、卵巢这些组织并没有嵌合。
- One EG cell line maintained undifferentiation for 6 passages. When bovine primordial germ cells were cultured on different feeder cells: MEF, STO and BEF, all can sustain bovine primary embryonic germ cells, and MEF was the best. 用不同的饲养层培养牛原始生殖细胞,实验结果表明MEF,STO和BEF饲养层都能支持牛原代PGCs的增殖,但MEF较BEF和STO效果更好以MEF、GEF、BEF为饲养层,在不添加细胞因子情况下,都可以培养出原代山羊的胚胎生殖细胞。
- Human EG cells possessed SSEA-1, SSEA-4, TRA-1-60, TRA-1-81, OCT4, C-kit and hTERT markers by immunohistochemistry in this study. The EG cells were AKP positive. OCT4 and hTERT expression of the EG cells could be tested by RT-PCR. 通过AKP染色、免疫组化染色和RT-PCR方法检测,人EG细胞特异性表达AKP、OCT4、SSEA-1、SSEA-3、SSEA-4、TRA-1-60、TRA-1-81、C-kit,和hTERT,这些研究丰富了人EG细胞生物学特性和检测方法。
- Thus, EBs provide a rich source of normal developing cell populations with both embryonic and adult phenotypes.Given this potential, what opportunities do human ES and EG cell lines offer? 正是这种能力发展成为一个广泛的细胞类型,已引起这么多关注的胚胎干细胞的基础研究工具,并作为一种新型的细胞群的新的临床治疗。
- We'll miss out (eg not sing) the last two verses. 最後两句歌词我们不唱了。
- Doors open (eg of a theatre) at 7.00 pm. 下午7时开门(如剧院).
- Human tissue is made up of cells. 人体的组织是由细胞构成的。
- Her new album has two great tracks (eg songs) on it. 她的新唱片集里有两段(如歌曲)特别棒。
- Have you got a light? eg for acigarette. 你有火儿吗? (如为点香烟).
- A tissue is a group of similar cells which have an identical general function. 组织是一组具有相同的一般机能的类似细胞。
- They (eg The things you are carrying) go on the bottom shelf. 这些东西(如你拿著的)放在架子的底层上。
- Animal bodies are made up of cells. 动物的身体是由细胞组成的
- A change of air (eg a holiday away from home) will do you good. 换换环境(如离家度假)对你有好处。
- I took some light reading (eg a thriller) for the train journey. 我带上一些轻松的读物乘火车时消遣。
- A small mass of tissue or aggregation of cells. 结组织的小结块或细胞的聚集
- I don't like being desk-bound (eg in an office) all day. 我不喜欢整天拴在办公桌旁(如在办公室里).
- His house was broken into (eg by burglars) last week. 上星期有人闯入他的房屋(如窃贼).