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- An eukaryotic vector, which highly expresses calmodulin, was constructed by DNA recombination in vitro and transfected into NRK cells. 实验中运用DNA体外重组技术构建了高表达钙调素的真核载体,并将其转染到大鼠正常肾细胞(NRK)中得到钙调素高表达的稳定细胞株。
- Keywords human endostatin;DNA recombination;gene overexpression; 人内皮生长抑素;DNA重组;基因高效表达;
- DNA recombinant technology have brought innovation to the biolog y technology, it can supply the strains with strong selectivity and ability of accumulating target metabolites. 重组DNA技术使生物技术取得了彻底性的变革,它可以提供具有极强选择性和累积目的代谢物能力的菌株。
- Keywords DNA recombination;Chlamydomonas reinhardtii;wastewater treatment; DNA重组;莱茵衣藻;废水处理;
- Conclusion Cellular immunity in mice could be enhanced induced by DNA vaccines based on HPV16 E6/E7. It could be much stronger induced by DNA recombinants of HSP70 of mycobacterium tuberculosis and HPV16 E6/E7. However, humoral immunity remained. 与结核杆菌HSP70重组后,IL-10及抗体水平没有明显变化。 结论以HPV16 E6/E7为基础的DNA免疫能增强小鼠的细胞免疫反应。
- Methods The pCI neo KH ORF expression vector was constructed by DNA recombinant technique and was introduced into COS 7 cells and K562 cells by lipofectactin mediated DNA transfection. Expression of KH ORF mRNA was detected by RT PCR. 方法 采用DNA重组技术构建KH ORF的表达载体pCI neo KH ORF ,通过脂质体法转染COS 7细胞和K5 6 2细胞 ,RT PCR检测目的基因的表达。
- Methods:The HIF-dependent recombinant adenovirus Ad-5HRE/hCMVmp-BCD was constructed by DNA recombinant technique. Western blot was used to detect HIF-dependent expression of bacterial cytosine deaminase (BCD). 方法借助DNA重组技术构建HIF依赖性表达的重组腺病毒载体Ad-5HRE/hCMVmp-BCD。
- Keywords NT3;Retroviral Vector;DNA Recombinant; 关键词NT3;逆转录病毒载体;基因重组;
- An enzyme that catalyzes genetic recombination. 催化重级酶能催化基因重组的一种酶
- DNA fingerprinting can help the police to find the criminals. DNA指纹术有助於警察发现罪犯。
- DNA recombination DNA重组
- DNA recombination role DNA重组作用
- The mutant DNA miscoded a single amino acid. 突变体脱氧核糖核酸误编了一个氨基酸密码。
- The committee recommended that recombination DNA work at Princeton be considered in this same light. 委员会建议用同样的观点来考虑在普林斯顿的重组体。
- Your DNA is in every part of your body. 你的dna存在于身体的各个部分。
- DNA is a self - replicating molecule. 脱氧核糖核酸是一种自我复制的分子
- The degree of recombination is reciprocal in most cases. 重新结合的程度在大多数情况下是相互的。
- Each clone would have the same DNA. 每一个克隆人都有着同样的dna。
- We can only make clones from living DNA. 我们只能用活的dna来克隆人。
- Extract the genomic DNA of a BLAB/c mice . 用酚氯仿异戊醇抽提blab/c鼠全基因组DNA;