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- DEAE Sephacel 二乙氨乙基葡聚糖纤维素
- After purification with DEAE Sepharose F. 结果：成功得到海螵蛸粗多糖。
- Methods: The polysaccharide was got by the extraction, DEAE chromatography etc. 方法:依次用水提取、过滤、去蛋白、DEAE纤维素层析、冷冻干燥获得大蒜多糖;
- For DEAE Sepharose FF weak anion-exchange resin chromatography, the optimum operation was with buffer Tris-HCl(Ccl_ =20mM)，pH8.0 and the concentration of NaCl was also 0.4M. 对于DEAE弱阴离子交换层析柱，采用Tris-HCl(Ccl_ =20mM)，pH8.;0缓冲体系;同时洗脱缓冲体系也维持NaCl浓度为0
- A technique of purified human albumin from the FIV of cold ethanol method was studied. The precipitate of FIV was dissolved and filtrated, then purified by DEAE Sephadex FF and CM Sephadex FF Chromatography. The final product was in ratter high purity. 以低温乙醇法分离血浆蛋白过程中的 FIV组份为原料 ,经 DEAE和 CM两步离子交换层析法 ,可制备出高纯度的人血白蛋白 ,该制品不含多聚体。
- A three-step column chromatographic method,utilizing CM Sepharose CL-6B,DEAE Sepharose Fast Flow and Sephadex G-75 in sequence,for the separation and purification of thrombin-like enzyme from crude venom of Agkistrodon halys pallas was developed. 建立了一种用CM Sepharo se CL-6B阳离子交换、DEAE Sepharo se Fast F low阴离子交换和Sephadex G-75凝胶排阻三步柱色谱从江浙蝮蛇蛇毒中分离纯化类凝血酶的方法。
- A trypsin like enzyme(AGP, Mr 14kD) was purified after ammonium sulfate precipitation, Sephadex G 75 gel filtration, Sephadex DEAE A 25 ion exchange chromatography and SBBI Sepharose 4B affinity chromatography. 肠液经硫铵沉淀，SephadexG?75凝胶过滤，SephadexDEAEA?25离子交换和SBBI?Sepharose4B亲和层析，分离纯化出分子量约为14kD的巨尾阿丽蝇蛋白酶。
- After transfection of pPTA1/Ig into COS 7 cells by DEAE dextran, expression of a secreting fusion protein was identified by affinity chromatography and sandwich ELISA assay with anti PTA1 mAb and HRP anti hIgFc mAb. 重组载体通过DEAE?dextran法转染COS?7细胞，经夹心ELISA及亲和层析、SDS?PAGE鉴定融合蛋白的表达及免疫学活性。
- Methods: EPF was purified from 430ml human pregnancy sera. The purification scheme involved sequential DEAE 52 chromatography, SP Sepharose F. F chromatography, ConA Sepharose 4B chromatography and Heparin Sepharose C1-6B chromatography. 方法:从430ml早孕血清中采用柱层析方法分离纯化EPF,依次经过DEAE 52阴离子交换层析、SP Sepharose EF阳离子交换层析、ConA Sepharose 4B亲和层析和Heparin Sepharose C1-6B亲和层析。
- Another glycoprotein elicitor MGJ2 was purified utilizing Sephacryal-100 gel filtration, DEAE sepharose FF ion exchange, SDS-PAGE showed that the MGJ2 had only one band of 102.2 kDa. 3 将稻瘟菌粗激发子经S-100凝胶柱层析，在全排阻阶段，收集活性峰，而后经DEAE-Sepharose FF离子交换柱层析获得糖蛋白激发子MGJ2，分别经SDS-PAGE蛋白银染及高碘酸-Schiff糖染色均显示单一条带，表明MGJ2激发子达到电泳纯。
- DEAE dextrandiethylaminoethyl dex-tran 二乙氨乙基葡聚精
- Keywords MSFB;pellicular adsorbent;Agarose gels;BSA;DEAE; 磁稳流化床;核壳型吸附剂;琼脂糖凝胶;BSA;DEAE;
- Keywords Konjac glucomannan;DEAE;Anion exchanger;Exchange capacity; 魔芋葡甘聚糖;DEAE;阴离子交换介质;离子交换容量;
- Keywords milk;food allergen;immunoblotting;DEAE iron-exchanged chromatography; 牛乳;食物过敏原;免疫印迹;DEAE离子交换层析;
- Sephaceln. 葡聚糖纤维素
- Study on Adsorption Performance of BSA in Streamline DEAE and DEAE Sepharose FF Adsorbents 蛋白质在膨胀床吸附层析剂的静态和动态吸附性能
- Keywords Streamline DEAE;Prothrombin Complex Concentrate（PCC）;ion-exchange adsorption;adsorption equilibrium; 人凝血酶原复合物;离子交换吸附;吸附平衡;
- Keywords Methylomonas Z201;MMO;DEAE cellulose;Covalently immobilization;Propene epoxidation; 甲烷单加氧酶;DEAE纤维素;共价固定化;丙烯环氧化;
- ISOLATION AND PIRIFICATION OF ANTICOAGULANT POLYPEPTIDES BY DEAE ION EXCHANGE CHROMATOGRAPHY AND SEPHADEX GEL FILTRATION 离子交换层析和凝胶过滤分离纯化抗凝血多腚
- Keywords Lactobacillus plantarum;lactate dehydrogenase;DEAE chromatography;hydrophobic chromatography;enzyme catalytic reaction; 植物乳杆菌;乳酸脱氢酶;DEAE层析;疏水层析;酶促反应条件;
