A three-step column chromatographic method,utilizing CM Sepharose CL-6B,DEAE Sepharose Fast Flow and Sephadex G-75 in sequence,for the separation and purification of thrombin-like enzyme from crude venom of Agkistrodon halys pallas was developed.

• 建立了一种用CM Sepharo se CL-6B阳离子交换、DEAE Sepharo se Fast F low阴离子交换和Sephadex G-75凝胶排阻三步柱色谱从江浙蝮蛇蛇毒中分离纯化类凝血酶的方法。