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- Culture supernatant fluid 培养上清液
- Quantibody arrays can be used in cell culture supernatant, serum, plasma, CSF, tissue lysate, and other body fluid. Quantibody芯片可用于培养细胞上清、血清、血浆、集落刺激因子、组织匀浆以及其他生物学液体。
- The carcinogenetic and tumor-promoting effects of concentrated Helicobacter pylori culture supernatant in rats. 幽门螺杆菌培养滤液诱癌和促癌作用的实验研究。
- The Ig class of McAb was identified by the indirect ELISA using the serum-free culture supernatant McAb. 用无血清型单抗作间接ELISA测定单抗的Ig类别。
- It is reported that, the culture supernatant of Toxoplasmagondii injected into the peritoneal can induce ab. 有报道称,用弓形虫培养上清注射到孕鼠体内也可以引起流产的发生。
- Objective: To study the growth factor like action of the Sika Deer splenocytes culture supernatant. 目的:测定梅花鹿脾细胞培养上清的生长因子样活性。
- Objective To investigate the carcinogenetic and tumor promoting effect of concentrated Helicobacter pylori culture supernatant(CHCS). 目的观察大鼠胃粘膜在幽门螺杆菌(Hp)培养滤液长期作用下的组织学变化及其对大鼠实验性胃癌的促癌作用。
- SDS-PAGE results indicated that there was a clear target band in Muts and Mut+ recombinant Culture supernatant after 48 hours culture respectively. 实验结果表明,诱导表达48h后,Mut+和MutS重组子表达的产物在SDS-PAGE胶上都出现了清晰的目的带。
- The effect of concentrated H. pylori culture supernatant (CHCS) on unscheduled DNA synthesis (UDS) in human gastric mucosal cells was studied. 本实验以布氏肉汤旋转培养法制备幽门螺杆菌提取液,观察其对胃粘膜细胞非程序DNA合成的影响。 结果表明幽门螺杆菌提取液可诱导胃粘膜细胞的UDS。
- The activity of this reconstructed system was found to be sensitive to amytal and DNP. The soluble factor in the supernatant fluid required for the energy-linked NAD~+ reduction was unstable. 由上清液所促进的NAD~+需能还原也同样地被Amytal与DNP所抑制。
- The time of harvest can be decided by measuring culture pH value and the culture supernatant containing rich PT and FHA can be gained. 通过测定培养液pH值以确定收获时间,可获得富含PT、FHA、且活性均保持较高水平的培养液
- Objective To explain the role of mitochondrial pathway in the apoptosis of SGC-7901 cell line induced by concentrated Helecobacter pylori culture supernatant (CHCS). 目的研究线粒体途径在幽门螺杆菌(Hp)诱导胃癌细胞凋亡过程中的作用。
- Collagen content of CFs culture supernatant was estimated by hydroxyproline chromatogragy. Nitrate enzyme reverting method was also adopted to evaluate NO content. 方法采用胶原酶消化法培养SHR和WKY的CFs,羟脯氨酸比色法测定CFs培养上清胶原含量,硝酸还原酶法测定CFs培养上清NO浓度,分光光度计法测定CFs培养上清NOS活性。
- AIMS To assess the effect of concentrated Helicobacter pylori culture supernatant (CHCS) on cell kinetics and unscheduled DNA synthesis (UDS) in rat gastric mucosa. 目的 观察Hp代谢物对大鼠胃粘膜细胞动力学和非程序DNA合成的影响。
- Results: The Sika Deer splenocytes culture supernatant has action on the proliferation of NIH3T3 cells,and also can promote the wound healing of NIH3T3 cells. 结果:梅花鹿脾细胞培养上清对正常小鼠NIH3T3细胞具有促增殖作用,对创伤的NIH3T3细胞具有促进愈合作用,并且在无血清培养液中此上清对正常NIH3T3细胞也具有促增殖作用。
- However, the cytolysin activity in the culture supernatant of the PD mutant was found to be twice as that of the wild-type strain and maintained for a much longer period. 此外PD突变株的培养上清液中其细胞溶解毒素的活性要比野生株高出一倍而且作用亦能持续一段时期。
- Methods: The culture supernatant of cord blood LAK cells and diluted E.coli suspension were cocultured in agar medium and the colonies of E.coli were counted. 方法将脐血LAK细胞培养上清液和稀释的埃希氏大肠杆菌悬液混合,在培养皿内培养不同时间,计数各个培养皿内细菌菌落数。
- CFs number was measured by MTT assay. Collagen content of CFs culture supernatant was estimated by hydroxyproline chromatogragy. Nitrate enzyme reverting method was adopted to evaluate NO content. 方法采用胶原酶消化法培养Wistar大鼠的CFs、四氮唑蓝(MTT)比色法测定CFs的增殖情况、羟脯氨酸比色法测定CFs培养上清胶原含量、硝酸还原酶法测定CFs培养上清NO含量、分光光度计法测定CFs培养上清NOS活性。
- The effects of cultured supernatant of fibroblast from psoriatic lesion or normal skin on keratinocytes were determined by MTT colorimetric methods. 结果 银屑病成纤维细胞培养上清培养的角质形成细胞的增殖活性高于正常人成纤维细胞培养上清组和无血清培养的角质形成细胞。