Collagen content of CFs culture supernatant was estimated by hydroxyproline chromatogragy. Nitrate enzyme reverting method was also adopted to evaluate NO content.

• 方法采用胶原酶消化法培养SHR和WKY的CFs,羟脯氨酸比色法测定CFs培养上清胶原含量,硝酸还原酶法测定CFs培养上清NO浓度,分光光度计法测定CFs培养上清NOS活性。