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- Aim: To observe if bcl-2 antisense oligodeoxynucleotide (ASODN) mediated by jetPEI-RGD improved effect on colon carcinoma cell line caco-2. 目的:观察聚乙烯亚胺(PEI)-整合素蛋白的配体(Arg-Gly-Asp, RGD)介导的bcl-2反义核酸(antisense oligodeoxynucleotide, ASODN)对结肠癌细胞caco-2的作用效果。
- AIM To observe the combination effects of 5-fluorouracil (5-FU) and curcumin in human colon carcinoma cell line HT-29 in vitro. 摘要 目的 体外观察5-氟尿嘧啶与姜黄素联用对人结肠癌HT-29细胞增殖的相互作用。
- Methods pCR3.1/GR plasmid expressing gastrin receptor CCK 2R was transfected into colonic carcinoma cell line CoLo320 by Lipofectamine~(TM) 2000 and expressing stably CCK 2R clones was screened by G418.The expression levels of gastrin receptor of Coi. 方法脂质体转染表达胃泌素受体CCK-2R的pCR3.;1/GR质粒于结肠癌细胞CoLo320中;G418筛选出稳定表达CCK-2R的阳性克隆;RT-PCR鉴定;转染成功命为CoLo320WT。
- Methods:An ADM-resistant human colon carcinoma cell line SW480/ADM was induced by continuously exposing human colon carcinoma cell line SW480 to gradually increasing doses of Adriamycin(ADM). 方法:应用人结肠癌细胞系SW480,以递增阿霉素(ADM)浓度的方法,体外连续培养建成一株SW480/ADM。观察该细胞生长规律。
- Human colon carcinoma cell line HT-29 结肠癌细胞系T29
- Successful construction of the recombinant adenovirus vector containing the Ki67-siRNA gene was achieved and it can be used to significantly inhibit expression of the Ki67 gene and the growth human colon carcinoma cells. 含有Ki67-siRNA的重组腺病毒构建成功,其能抑制人结肠癌细胞Ki67基因表达和生长。
- Objective:To investigate the combined anti-tumor effects of apoptin gene,hemagglutinin-neuramidinase gene(HN)of newcastle disease virus(NDV),and human IL18 gene(hIL-18)on colon carcinoma cells HCT-116 in vitro. 目的:探讨联合应用凋亡素(apoptin)基因、新城疫病毒(newcastle disease virus,NDV)血凝素-神经氨酸酶(he-magglutinin-neuramidinase,HN)基因及人白介素18(hIL-18)基因体外对人结肠癌细胞HCT-116的抑制效应。
- Methods:The recombinant plasmid pIRApoptinHNIL18 was introduced into human colon carcinoma cells HCT-116 by liposome-mediated transfection. The expression of the exogenous genes was detected by Western blotting and RT-PCR. 方法:重组质粒pIRApoptinHNIL18通过脂质体介导法体外转染人结肠癌细胞HCT-116,通过Western blotting和RT-PCR检测外源基因表达;
- Expression of IL-6 in Transferred Human Colon Carcinoma cell Line IL-6基因转导大肠癌细胞的表达
- Keywords Apoptin;EGFP;Human colonic carcinoma cell line;Cell apoptosis; 关键词凋亡素;增强型绿色荧光蛋白;人结肠癌;细胞凋亡;
- Expression of HSP70 in human colon carcinoma cells HSP70在大肠癌细胞的表达规律
- Effect of Down-regulating VEGF on Proliferation of Colon Carcinoma Cell HT-29 抑制VEGF基因表达对大肠癌细胞HT-29的增殖影响
- Such occurs with the patient of colonic carcinoma. 此情况见于结肠癌病人。
- Studies of the effects of recombinant human tumor necrosis factor on human colon carcinoma cell line SW-480 in vitro 重组人肿瘤坏死因子对人结肠癌SW-480细胞在体外的杀伤作用研究
- Keywords Elemene;Colon carcinoma cell line Lovo;Telomerase activity;Apoptosis;Cell cycle; 关键词榄香烯乳;结肠癌细胞;端粒酶活性;细胞凋亡;细胞周期;
- Effects of matrix metalloproteinase-9 on the expression of tumor necrosis factor receptor-1 in human colon carcinoma cell lines 基质金属蛋白酶-9对大肠癌细胞肿瘤坏死因子受体1表达的影响
- Transfection and anti-tumor effects of APO-1 gene in colon carcinoma cells APO-1基因转染及其抑制大肠癌细胞生长的实验研究
- Keywords calcium channel activity;pentagastrin(PG);cell proliferation;intracellular free calcium;human colon carcinoma cell line; 钙通道活性;五肽胃泌素;细胞增殖;细胞内游离钙离子;人结肠癌细胞株;
- Keywords calcium channel activity;pentagastrin;cell proliferation;intracellular free calcium;human colon carcinoma cell line; 钙通道活性;五肽胃泌素;细胞增殖;细胞内游离钙离子;人结肠癌细胞株;
- There was different NMPs in different types of colon carcinoma. 在不同类型大肠癌中,核基质蛋白表达也具有差异。