Methods pCR3.1/GR plasmid expressing gastrin receptor CCK 2R was transfected into colonic carcinoma cell line CoLo320 by Lipofectamine~(TM) 2000 and expressing stably CCK 2R clones was screened by G418.The expression levels of gastrin receptor of Coi.

• 方法脂质体转染表达胃泌素受体CCK-2R的pCR3.；1/GR质粒于结肠癌细胞CoLo320中；G418筛选出稳定表达CCK-2R的阳性克隆；RT-PCR鉴定；转染成功命为CoLo320WT。