您要查找的是不是:
- Therefore, Bradford method was a good, rapid determination of protein content. 所以考马斯亮蓝法是一种很好的蛋白质快速测定方法。
- Quantitative analysis of protein adsorption was done by Bradford method. Bradford方法定量分析明胶、胶原和RGD多肽的表面吸附量。
- The concentration of extracted proteins were determined by Bradford method. 以Bradford测定法绘制蛋白定量标准曲线,对肝脏组织样品蛋白提取物定量;
- The purity and concentration were measured by 12% SDS-PAGE and Bradford method,then verified protein with ELISA. 通过12%25SDS-PAGE胶和Bradford法检测其纯度和目的蛋白含量,用ELISA对纯化后的蛋白进行鉴定。
- The concentration of cellular memberane protein was measured by Bradford method and the final concentration was modulated above Img/ml. 用Bradford法测定细胞膜蛋白质浓度,膜蛋白最终浓度控制在1mg/ml以上。
- IgY was isolated by using the water dilution method (WD) ,and their concentration and purity were measured respectively with Bradford method and SDS-PAGE. 用rHpaA免疫30周龄立克体鸡,以水稀释法联合盐析法提取HpaA-IgY,纯化浓缩后用Bradford法测定其含量,SDS-PAGE电泳检测纯度,Western blot鉴定抗原特异性。
- The results of determination for three serum samples were identical with those obtained according to the Bradford method using Coomassie Brilliant Blue (CBB G-250). 本方法成功应用于三个血清样品中蛋白质总量的测定,其结果与考马斯亮蓝法(Coomassie Brilliant Blue,CBB G-250)测定结果一致。
- In order to clarify the diversity and the variation of salivary gland proteins of female Der-macentor silvarum , the proteins were analyzed by Bradford method and SDS-PAGE. 为揭示森林革蜱在吸血过程中唾液腺蛋白的成分与变化特点, 本文应用Bradford法和SDS-PAGE对森林革蜱雌蜱唾液腺蛋白进行了研究。
- Secondly,differential protein concentration in the hemolymph and midgut of An. stephensi adult females fed on different meals was examined with Bradford method. 利用Bradford法测定不同食源条件下斯氏按蚊雌蚊血淋巴和中肠蛋白浓度差异。
- The Bradford method was used to measure the concentration of protein in liver microsome, and activities of cytochrome P450 enzymes were determined by the spectrophotometer method. 测定一月龄至八月龄不同性别的猪,肝微粒体蛋白含量、CYP450含量、CYPb5含量、NADPH-细胞色素C还原酶活性、氨基比林-N-脱甲基酶活性、红霉素-N-脱甲基酶活性和苯胺-4-羟化酶活性。
- An increase of protein concentration in the suspension, measured by the Bradford method, will then be used to confirm the cellular rupture of this yeast exposed in plasma. 考马斯亮蓝染色法测量细胞外蛋白质浓度发现,随等离子体处理时间的延长,胞外蛋白质的含量呈上升趋势,证明细胞内蛋白质发生泄漏。
- Using HiTrap Anti-E Tag column, the scFvs4-16 were purified by affinity chromatography. SDS-polyacrylamide gel electrophoresis of purified scFvs was analyzed. The consistencies and bioactivity of purified scFvs4-16 were measured by Bradford method. 制备大量抗肝癌scFv4-16,利用HiTrap Anti-E Tag亲和层析柱对表达的scFv4-16进行纯化;
- Hepama-1 concentration was above 2.50mg/ml by Bradford method, the area of mixed protein and polymer on the gel was less than 5% of total area of the isolated peak of immunoglubulin by SDS-PAGE electrophoresis. 经SDS-聚丙烯酰胺凝胶电泳及电泳胶扫描积分计算, 其杂蛋白及多聚体的面积小于蛋白分离峰总面积的 5%25;
- Lots of high-titer IgY were mixed andpurified by the ammonium sulphate precipitation ,its purity was identifiedby SDS-PAGE , titer and protein concentration were measured by indirectELISA and Bradford method. 用建立的检测 VacA-IgY 的 ELISA 法测定 IgY 产生的时间-抗体效价变化情况。 收集不同免疫时间的 VacA-IgY,混合后用硫酸铵沉淀法纯化浓缩,SDS-PAGE 分析其纯度。
- Cultural characteristics of Frankia The cultural characteristics, and effects of carbon and nitrogen sources, pH, temperature on three Frankia strains were studied by microscopic examination and protein content detected by modified Bradford method. Frankia菌培养特性的研究。 通过形态观察和Bradford改良法测定菌体蛋白含量,研究了3个菌株的培养特性和碳源、氮源、pH值及温度对它们生长的影响。
- Three detecting methods in yeast proteinase A (PrA) assay including UV absorbance, Lowry method and modified Bradford method were compared when using casein or hemoglobin . 酵母蛋白酶A是啤酒酵母中的一种蛋白水解酶,啤酒中残留的酵母蛋白酶A对啤酒泡沫有负面作用。
- The method seems good but it needs to be tried out. 这个方法似乎不错,但需要试验一下。
- He represents the people of Bradford in Parliament. 他在国会中代表布拉德福选区的人民。
- WSSV(white spot syndrome virus) is isolated from infected crayfish cambarus clarki. , by different speed centrifugation , sucrose cushion , sucrose gradient centrifugation. Quantified by Bradford methods, the concentration of WSSV is 0.912mg/ml. 使用差速离心、蔗糖垫和蔗糖密度梯度离心法,从人工感染白斑综合症病毒(White spot syndrome virus,WSSV)的克氏原螯虾中提取WSSV,Bradford法测定病毒浓度为0.;912mg/ml。
- Three detecting methods in yeast proteinase A (PrA) assay including UV absorbance, Lowry method and modified Bradford method were compared when using casein or hemoglobin as substrates. 其中以蛋白质为底物时的酶活性测定方法方便快捷,但是当酵母蛋白酶A酶活性较低时,产物采用紫外吸收法以及Lowry法的检测灵敏度都比较低。