Lots of high-titer IgY were mixed andpurified by the ammonium sulphate precipitation ,its purity was identifiedby SDS-PAGE , titer and protein concentration were measured by indirectELISA and Bradford method.

• 用建立的检测 VacA-IgY 的 ELISA 法测定 IgY 产生的时间-抗体效价变化情况。 收集不同免疫时间的 VacA-IgY,混合后用硫酸铵沉淀法纯化浓缩,SDS-PAGE 分析其纯度。