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- 16S rDNA gene frgement rDNA基因片段
- The agreement between Dot-ELISA and skim milk plate, Dot-ELISA and aer gene PCR, Dot-ELISA and 16S rDNA gene PCR were 79.2%(57/72), 91.6%(66/72), 81.9%(59/72) respectively. Dot-ELISA与脱脂奶平板法两者符合率为79.;2%25(57/72);与aer基因扩增的符合率为91
- Studies on diversity and phylogeny of slow-growing rhizobia isolated from Vigna radiata in China was conducted by using phynotype,16S rDNA gene PCR-RFLP , 16S rDNA gene sequencing, 16S-23S rDNA IGS PCR-RFLP and RAPD assays. 本研究采用表型性状测定、16S rDNA-RFLP、16S rDNA序列分析、16S-23S rDNAIGS RFLP分析以及RAPD分析等技术系统首次研究了从我国不同地域分离的44株绿豆根瘤菌的遗传多样性及其在根瘤菌系统发育中的地位和相互关系。
- 16S rDNA gene sequence 胞内微生物
- On the basis of homology and phylognetic analysis about 16S rRNA gene (16S rDNA), it could be speculated that the strain AB1 is a novel member of the genus Natronococcus. 基于16S rDNA序列的同源性比较及系统发育学研究表明，AB1是Natronococcus属中新成员。
- The result is the same to the sequnce result of 16S rDNA PCR amplification. 鉴定结果与16S rDNA基因PCR扩增测序鉴定结果一致，同时也验证了细菌分子鉴定的可靠性。
- The ribosomal internal transcribed spacer regions (ITS 1 and ITS 2) and mitochondrial gene fragments ( 16S rDNA and COI) of Crassostrea gigas were amplified via PCR, and the PCR products were ligated into T vectors, cloned and sequenced. 以相应引物经PCR扩增了太平洋牡蛎 (Crassostreagigas)的核糖体转录间区域 (ITS 1和ITS 2 )及线粒体 16SrDNA和COI基因片段。 PCR产物经T 载体连接后进行克隆和测序 ,分别得到长度为 5 4 3、791、5 30和 70 0bp的核苷酸序列。
- Based on the diagnosis kit for pathogenic Aeromonas, the ECPase54 produced by Aeromonas hydrophila (Ah) was detected by Dot-ELISA, at the same time the gene for specific 16S rDNA and aerolysin of pathogenic Aeromonas hydrophila (PAh) were detected. 在鱼类致病性气单胞菌诊断试剂盒的基础上,用Dot-ELISA检测致病性嗜水气单胞菌(Aeromonas hydrophila,Ah)胞外蛋白酶,同时扩增Ah的16S rDNA和aer基因。
- Compared with 16S rDNA PCR-RFLP, the result of IGS RFLP showed more differences among strains. IGS PCR-RFLP分析结果表明,供试菌株在70%25水平上分成6个遗传群,与16S rDNA RFLP分析结果比较,IGS RFLP反映的多样性更明显,形成的遗传群较多,可用于菌株的鉴别;
- At the same time,the composition of the SFC-2 was also analyzed by constructing 16S rDNA clone library. 通过16S rDNA克隆文库分析获得7个克隆;其近缘种主要为L.
- The result of 16S rDNA PCR-RFLP showed good agreement with that of numerical taxonomy. RFLP的聚类结果与数值分类的聚类结果有很好的一致性;
- In 122 subgingival plaque samples, the positive rates of 16S rDNA, lktA and fap genes by PCR were 84.4%, 75.4% and 50.0% respectively. Different A. 12 2份龈下菌斑中Aa 16SrDNA、lktA和fap检测阳性率分别为 84 .;4%25、75
- Sixty-seven strains isolated from the root nodules of Trifolium, Crotalaria and Mimosa in comparison with 18 reference strains were analysed by 16S rDNA PCR-RFLP. 采用 16SrDNAPCR -RFLP分析和表型数值分类 ,对分离自三叶草 (Trifolium)、猪屎豆 (Crotalaria)和含羞草(mimosa) 3属植物的根瘤菌进行了分类研究。
- The strain with the maximum enzyme activity level was a Gram negative bacterium which was identified as Stenotrophomonas maltophilia chitinase strain by 16S rDNA method. 对该菌株应用16S rDNA法进行鉴定;结果为嗜麦芽窄食单胞菌(Stenotrophomonas maltophilia).
- Method Evaluated by ERIC-PCR(Enterobacterial Repetitive Intergenic Consens-PCR) and PCR-TGGE(PCR-temperature gradient gel electrophoresis) of 16S rDNA V3 region. 方法利用ER IC-PCR基因组DNA指纹图技术和16S rDNA V 3扩增-温度梯度凝胶电泳(PCR-TGGE)指纹图技术。
- Qiu B S, Li H H, Shi C L, et al.Amplification of phytoplasma 16S rDNA from 20 infected plants in china and their RFLP analysis[J].Scientia Silvae Sinicae, 1998, 34(6): 67-74. [6]邱并生; 李横虹; 史春霖; 等.;从我国20种感病植物中扩增植原体16S rDNA片段及其RFLP分析[J]
- The average content of T, C, A, G of 28S rDNA gene sequence of 22 species of Tettigonioidea are 21.4%, 27.4% 17.7% and 33.5% respectively, and G+C content is 60.9%, G+C content is higher than A+T content obviously, which shows a strong G+C bias. A+T的含量明显高于G+C的含量。
- It was also evident that strain 12202 formed a distinct phyletic line with 5 strains of an as-yet-unnamed novel family ("Ellin5034 group"), sharing the highest 16S rDNA sequence similarity of 99.4%. 在以16S rDNA序列为基础的系统发育树上，菌株12202和一个迄今尚未定名、尚未生效发表的新科 (“Ellin5034 group”)的5个成员形成紧密相关的单独分枝，菌株12202的16S rDNA序列与该新科成员的序列相似性最高达99.;4%25。
- These strains were classified into the genus of Rhizobium Frank and Agrobacterium Conn according to their morphology, physiological and biochemical characteristics and analysis of 16S rDNA sequence. 根据形态特征观察、生理生化特征测定和16Sr DNA序列分析结果，将As-1、As-2和As-3菌株鉴定为根瘤菌属Rhizobium Frank，Ag-1菌株鉴定为土壤杆菌属Agrobacterium Conn。
- Except for IAM13129, B33 and 711, at the similarity level of 75% dendrogram derived from 16S rDNA RFLP-PCR showed that all isolates were devided into two phylogenetic branches: Rhizobium and Sinorhizobum and unknown one. 在75%25的相似水平上，除了IAM13129，B33，711，所有供试菌株全部聚群，分为Rhizobium和Sinorhizobum系统发育分支和未知系统发育分支。
