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- 载体pVAX1pVAX1 vector
- 将HeB02株F基因插入真核表达载体pVAX1中,构建了真核表达质粒pSV-F,通过脂质体转染COS-7细胞,SDS-PAGE分析可见表达的特异蛋白条带;The expression plasmid pSV-F was constructed by inserting the F gene into the pVAX1 vector, and transfected into the cultured COS 7 cell line via liposomes.
- 载体carrier
- 方法用PCR法从HCMV基因组中钓取gB680和pp65m蛋白基因,分别亚克隆入pMD18-T载体中,经酶切鉴定并测序后,构建真核表达载体pVAX1/gB680+pp65m,以ELISA法检测其在COS-7细胞中的瞬时表达。Methods Amplify gB680 and pp65m genes from genome of HCMV by PCR and subclone to vector pMD18-T respectively. After identification of the constructed recombinant plasmids by restriction analysis and sequencing,clone gB680 and pp65m genes into plasmid pVAX1.Transfect COS-7 cells with the constructed eukaryotic expression vector pVAX1/gB680+pp65m,and identify the expressed product by ELISA.
- 将SL7207(pVAX1-F)以109CFU/只剂量口服免疫1日龄雏鸡,免疫保护试验结果显示,SL7207(pVAX1-F)免疫组对鸡具有良好的保护率(77.27%),与空白对照组和SL7207(pVAX1)空载体组之间存在显著性差异(P<0.05)。The chickens immunized with SL7207(pVAX1-F) could be protected with the protective rate of 77.27%25,significantly higher than those with SL7207(pVAX1)(29.42%25) or PBS(12.5%25)(P<0.05).
- 承载体supporting body
- 表达载体expression vector
- 杆菌载体bacillus carrier
- 硅胶载体silica-gel carrier
- 白色载体white support
- 氧化铝载体alumina supporter
- 薄壳载体pellicular support
- 信息载体semantide
- 氨基酸转运载体amino acid carrier
- 卤载体halogen carrier
- 氯载体chlorine carrier
- 白色硅藻载体Chamelife
- 报导用载体announcement media
- 病毒载体virus vector
- 反载体hold-back carrier