您要查找的是不是：
- 然后将VP1基因分段克隆至原核表达载体pGEX-4T-1，经酶切及PCR鉴定，证明成功构建了重组表达载体pGEX-A、pGEX-B、pGEX-C。Based on the analysis results, three partition of VP1 gene of CAV were cloned and inserted into the bacterial plasmids pGEX-4T-1. The recombinant plasmids containing VP1 partiton gene of CAV were identified by restriction enzyme analysis and PCR method.
- 载体pGEXvector pGEX
- 载体carrier
- 结果构建了重组质粒pGEX-4T-CTB,CTB基因片段分子量约为376bp;Finally,the recombinant plasmid pGEX-4T-CTB was successfully constructed with a CTB gene fragment of 376 bps.
- PCR拼接法扩增壳聚糖酶基因并克隆入pGEM-Teasy载体进行序列分析,进一步亚克隆入表达载体pGEX-3X。coli favorites. The Csn gene was cloned into plasmid pGEM-Teasy and verified by DNA sequence analysis. Thereafter, Csn gene was subcloned into a fusion-protein expressing vector pGEX-3X.
- 将烟夜蛾的UBE基因克隆到原核表达载体pGEX-4T-2上,经IPTG诱导,SDS-PAGE电泳检测出约40kD的融合蛋白。The fragment containing UBE gene was inserted into pGEX-4T-2 expressive vector, and induced by IPTG. Molecular weight of ubiquitin fusion protein was about 40 kD by checking with SDS polyacrylamide gel electrophoresis.
- 承载体supporting body
- 表达载体expression vector
- 以克隆的激活蛋白基因为目的基因，构建了原核表达载体pGEX-XF-1，转化到大肠杆菌BL21和ER2566中，通过IPTG诱导，得到高效体外表达。Cloned activator protein gene was inserted into pGEX-5X-l to construct the recombinant prokaryotic expression vector, pGEX-XF-1. Then the vector was transformed into E. coli BL21 and ER2566. And high expression in vitro induced by IPTG was obtained and analyzed on SDS-PAGE gel.
- 杆菌载体bacillus carrier
- 葡萄球菌A蛋白（SPA）和链球菌G蛋白（SPG）的IgG结合区编码基因融合后，克隆到大肠杆菌表达载体PGEX，SPA/SPG融合蛋白（SPAG）得到高效表达。The encoding genes of IgG binding domains of staphyloccal protein A(SPA)nd streptococcal protein G (SPG)were cloned and expressed as fusion protein using pGEX vector of E. coli.
- 硅胶载体silica-gel carrier
- 白色载体white support
- 氧化铝载体alumina supporter
- 薄壳载体pellicular support
- 信息载体semantide
- 氨基酸转运载体amino acid carrier
- 卤载体halogen carrier
- 氯载体chlorine carrier
- 白色硅藻载体Chamelife
