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- 质粒pEGFP-N1pEGFP-N1
- 方法以质粒pEGFP-N1为模板扩增EGFP基因,EcoR I和Hind III双酶切EGFP基因序列和pET28a载体。Methods Using plasmid as template,full-length EGFP gene was amplified. The amplified EGFP gene and pET28a vector were cleaved by EcoR I and Hind III,then ligated after purifying.
- 将其克隆入pEGFP-H1/siRNA,构建表达EGFP及TLR4-shRNA的真核表达质粒pEGFP-H1/TLR4-siRNA。Then an oligo nuclear hairpin sequence targeting TLR4 gene was designed by the internet tool siRNA Wizard and then inserted into the plasmid pEGFP-H1/siRNA so as to form the plasmid pEGFP-H1/TLR4-siRNA.
- 方法:将壳聚糖纳米粒包裹的含报告基因的质粒pEGFP-C1分别转染前列腺癌细胞PC-3和22Rv1,并观察EGFP的表达情况。Methods:Prostate cancer cells PC-3 and 22Rvl were transfect- ed with plasmid pEGFP-C1 coated with chitosan nanoparticles and the expression of EGFP was observed.
- 观察差异表达SJCHGC蛋白重组真核表达质粒pEGFP-C1/SJCHGC在COS-7细胞中的表达和亚细胞定位,并分析其表达产物的抗原性.To observe the in vitro expression and subcellular localization of recombinant eukaryotic expression plasmid pEGFP-C1/ SJCHGC in COS-7 cells and analyze the antigenicity of the products expressed.
- 质粒plasmid
- 目的构建抑制大鼠心肌细胞k ir2.1基因的真核表达质粒pEGFP 6-k ir2.1,观察对大鼠心肌细胞k ir2.1信使RNA(mRNA)、蛋白表达情况及搏动频率的影响。Objective To construct the expression short hairpin RNA(shRNA) targeting gene kir2.1 in rat myocardial cells,named pEGFP6-kir2.1,and to observe the effects on the expression of messenger RNA(mRNA) and protein of gene kir2.1 as well as the changes of myocardial beating rates.
- pEGFP-N1质粒Plasmid pEGFP-N1
- 重组质粒dna recombinant plasmid
- R质粒R plasmid
- Ri质粒Ri-plasmid
- Ti质粒Ti-plasmid
- 胞质粒albuminous granules
- 不接合质粒nonconjugation plasmid
- ti 质粒ti plasmid
- 嵌合质粒chimeric plasmid
- 质粒工程plasmid engineering
- 质粒排除plasmid elimination
- 质粒转导plasmid transduction
- 自主质粒autonomous plasmid