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- 首先通过PCR的方法,将编码大鼠DAF四个SCR的基因片段亚克隆到Trx融合原核表达载体pET32a(+)。Firstly, I subcloned the DNA fragment that encode the four consensus repeat of DAF into the prokaryotic expression vector pET32a(+) which could add a Trx tag on the N-terminal of the target protein.
- 方法:采用RT-PCR技术从人肝癌细胞SMMC-7721中扩增出剪切掉线粒体定位信号的人AIF基因片段,并按阅读框克隆到原核表达载体pET32a(+)中。METHODS: A human AIF gene fragment of 1515 bp(mitochondrial localization sequence was deleted) was amplified from SMMC-7721 cells by reverse transcription-polymerase chain reaction (RT-PCR) and cloned into pET32a(+) vector to construct recombinant plasmid pET32a-AIF.
- 表达载体pET32aexpression vector pET32a
- 方法利用PCR技术扩增tlh基因,克隆至载体pET32a+并测序。 将测序结果提交国际互联网上有关生物信息网站进行生物信息学分析。Methods The tlh gene amplified by PCR was cloned into the vector pET32a+ and sequenced, followed by analysis of the biological information by with presenting the sequences to the websites of bioinformatics on the Internet.
- HBV全基因核心蛋白变异株稳定表达载体的构建及其抗原表达CONSTRUCTION OF STABLE EXPRESSION VECTORS WITH HBV GENOME CAPSID PROTEIN MUTANTS AND THEIR ANTIGENIC EXPRESSION
- 芳香烃受体及其核转运蛋白融合表达载体的构建与序列鉴定Construction and sequence analyzing of aryl hydrocarbon receptor and aryl hydrocarbon receptor nuclear translocator
- 共表达载体coexpression vector
- DNA表达载体DNA expression vecotor
- pET表达载体pET expression vector
- 表达载体法siRNA expression vectors
- 原核表达载体prokaryotic expression vector
- 表达载体构建Expression vector construction
- 基因表达载体gene expression
- RNAi表达载体RNAi expression vector
- 双元表达载体binary expression vector
- 酵母表达载体yeast expression vector
- pIL2表达载体pIL2 plasmid
- 质粒表达载体plasmid expression vector
- 高效表达载体High-efficient expression vector