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- 扩增后cDNA分级分离Amplified cDNA size fractionation
- 选择L2扩增后与原毒S1133株同时免疫1日龄雏鸡,免疫后15天脚垫攻击鸡呼肠弧强毒S1133株. 攻毒后20天。Chicks at 1-day of age were vaccinated with L2 strain and original S1133 strain separately.
- 将这些标本采用 PCR扩增后结合杂交重新检测，检出率为 94.7y，在肾移植并发 PCP患者的 BAL中检出 PC DNA。PCR combined with dot slot hybridization could detect PC from 94.7%25 of these samples, and PC DNA was detectable from the BAL of the patient.
- 旋风筒的分级分离效率与其入口风速的关系RELATION BETWEEN FRACTIONAL COLLECTION EFFICIENCY AND INLET VELOCITY OF CYCLONE
- cDNA扩增cDNA amplification
- 章鱼干糖蛋白的提取与分级分离的初步研究STUDY ON EXTRACTING AND FRACTIONATING OF GLYCOPROTEIN OF DRIED OCTOPUS
- cDNA扩增片段长度多态性（cDNA-AFLP）eDNA amplified fragment length polymorphism (cDNA-AFLP)
- 用垂直转头密度梯度超速离心法快速分级分离多聚核糖体Rapid fractionation of polysomes by density gradient ultracentrifugation in vertical tube rotor
- 扩增图谱amplification map
- 除了分析，膜免疫层析的原理还能延伸到化学样本的原位分级分离。Besides analysis, the concept of membrane immunochromatography can be expanded to in situ fractionation of chemical species.
- 扩增条带Amplified band
- 应用PCR技术扩增了MDPV_Q株的VP2 蛋白基因片段。 将扩增后的VP2 基因重组到pMD18_T质粒载体上 ,并对插入片段进行序列测定。The DNA encoding VP 2 structural protein was amplified by PCR and recombined into T vector and sequenced Compare gene and amino acid squence of VP 2 of MDVP_Q with that of GPV and MDPV which were registered in the genbank.
- 扩增和缺失expansion and contraction instability
- 多重耐药株以及敏感株acrAB基因扩增产物无点突变和缺失。No deletion or point mutation in acrAB gene were found in Mar and susceptible clinical Escherichia coli isolates.
- 全基因组扩增whole-genome amplification
- 方法应用PCR从乳腺文库中扩增得到HMGB1基因片段,并将其克隆到pET28a(+)载体上,对获得的pET28a(+)-HMGB1重组质粒扩增后进行PCR、酶切和测序鉴定。Methods Full length HMGB1 cDNA cloned by PCR from mammary library was inserted into pET28a(+) vectors to construct pET28a(+)-HMGB1 plasmid. After confirmation, the positive pET28a(+)-HMGB1 recombinant plasmid was transformed into E.
- 多重置换扩增multiple displacement amplification
- 环介导等温扩增Loop-mediated isothermal amplification
- 成环恒温扩增法loop-mediated isothermal amplification
- 三核苷酸重复扩增Trinucleotide repeat expansion
