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- virulence gene site 毒力位点
- The overall virulence gene prevalence was 62.7% (42/67); and the main virulence gene type was slt_1+ slt_2+eaeA+hly. 62.;7%25的株菌(42/67)携带有毒力基因;毒力图谱类型主要为slt1+slt2+eaeA+hly。
- By the colony hybridization with the virulence gene brobes of ctx, zot, ace, and RS1, all of CVC were positive. 用ctx、zot、ace、RS1为探针，对471株O1群霍乱弧菌进行毒力基因检测； 结果：CVC菌株全部阳性；
- Gene site DNA amplification was analyzed with polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). 进行基因位点DNA扩增聚合酶链反应-单链构象多态性分析。
- Also covered are methods for studying gene expression and detecting virulence genes. 此外包括是用于研究基因表示和检测有毒基因方法。
- These results indicate that the major virulence genotype of Anhui isolation strains is the high virulence phenotype of alt~+aha1~+aerA~+ and the virulence gene alt generally exists in different Aeromonas phenospecies. 表明气单胞菌安徽分离株的主要毒力基因型是alt+aha1+aerA+的高毒力表型,alt毒力基因普遍存在于不同表型种气单胞菌中。
- Adhesion gene (aha1), aerolysin gene (aerA) and cytotonic enterotoxin gene (alt) were considered as major virulence genes of Aeromonas. 粘附素(aha1)、气溶素(aerA)和细胞兴奋性肠毒素(alt)是气单胞菌的主要毒力因子。
- Methods By RT-PCR,specific amplification products of P2 gene from H2 final product and its parent seed were obtained,then sequenced and blasted to analyze its virulence gene VP1/2A and 2C. 方法通过逆转录-聚合酶链反应,特异扩增甲肝减毒活疫苗H2株成品疫苗及其亲本毒种P2区VP1/2A、2B、2C基因序列,经测序、比对,分析检测毒力基因VP1/2A、2C的变异情况。
- Some pathogenicity was steadily inherited, which suggested that the virulence genes controlling the pathogenicity appeared to be homozygous nuclear gene. 控制大豆疫霉菌的致病基因分布在不同位点，有的遗传稳定，由纯合的核基因控制；
- In 20 grade 1 ulcers (92%), no virulence genes were identified whereas these genes were present in all but one grade 2-4 ulcers. 除了1名病人外，致病基因在所有2-4级溃疡病人中都被检测到，而在1级溃疡病人中，20名(92%25)未检测出致病基因。
- On the contrary, two grade 1 ulcers healed: the genotype profiles were different from those at inclusion but without appearance of virulence genes. 相反，两名1级病人治愈后，基因型却不同于那些包含但未表现的致病基因。
- Pseudomonas is also known to switch on its virulence genes in response to signals from quorum sensing. 据了解假单细胞菌也会打开它的有毒基因以响应来自群体的信号。
- According to the four types of virulence genes: stx1, stx2, eaeA and hlyA, four pairs of specific primers have been designed. [方法]针对产志贺毒素大肠杆菌(STEC)的stx1、stx2、eaeA、hlyA4种毒力基因,设计了4对特异性引物。
- Methods Virulence genes, including stx1, stx2, EPEC and EHEC general eaeA fragment (eaeA-gen), were amplified by multiplex PCR in E. coli strains. 方法 应用多重PCR同时检测大肠埃希菌菌株的stx1、stx2、EPEC和EHEC共同的eaeA(eaeA -gen)等靶基因 ;
- There are also unknown virulence factors being identified with exception of known virulence genes identified in all these screens. 这些筛选中除了找到已知的毒力基因外,还都鉴定到了未知的毒力因子。
- Using oligonucleotide arrays, S. aureus resistance and virulence genes were compared between grade 1 and grade 2-4 ulcers. 应用寡核苷酸芯片后，比较1级和2-4级溃疡病人中，金黄色葡萄球菌的耐药性和致病基因。
- During follow-up, the two grade 1 ulcers which infected with strains carrying virulence genes rapidly deteriored: the array technology showed unchanged genotype profiles. 在随访中，感染携带致病基因的两名1级病人病程急剧恶化：芯片方法显示基因型未发生改变。
- Of forty eight isolates carrying virulence genes, 38( 79.2%) had SLT 2, eaeA and hly genes, taking the dominate virulence gene pattern, 8( 16.6%) had all of the four virulence genes 2( 4.2%)had both SLT 2 and hly genes respectively. 菌株毒力基因图谱以SLT2 +eaeA +hly为主 ;占 79.;2%25;其次为SLT2 +SLT1+eaeA +hly和SLT2 +hly;分别占 16
- Keywords S.typhi;R plasmid;Virulence gene; 伤寒杆菌;耐药质粒;毒力基因;
- The assay was designed to amplify the 1109, 302 and 228 bp regions of corresponding virulence genes eaeA, hlyAB, slt1 and /or slt2, by using specific primers for each one reaction. 将样品增菌后 ,采用快速裂解吸附法制备模板 ,用多重PCR同时检测EHEC的三种毒力基因eaeA、hlyAB、slt1 2 ,相应扩增片段依次为 110 9、30 2、2 2 8bp。 检测了 6 0株大肠杆菌和其它菌种。
