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- Viability of hepatocytes by both the trypan blue exclusion test and the ultrastructure of hepatocytes were determined. 而在编织型生物反应器内,肝细胞聚集体在相互交叉编织的纤维支架和尼龙膜的支撑下不易在其底部沉积;
- trypan blue exclusion test 台盼蓝不相容试验
- Methods The number of viable cells was ascertained by trypan blue dye exclusion test. 人们逐渐认识到肿瘤的生成是细胞增殖和凋亡平衡失调的结果。
- Results Trypan blue exclusion demonstrated >95 % viability. 结果台盼蓝染色,细胞存活率大于95%25;
- Method:Trypan blue exclusion, 3H|TdR incorporation and 3H|proline incorporation were assayed when LFb cultured in AM|conditioned medium on day 7 after bleomycin exposure. Western blot was carried out to determine the change of Ras process. 方法 :肺纤维化 1周组肺泡巨噬细胞 (AM)条件培养上清液与正常大鼠LFb共同培养 ,并给予不同浓度的Sim干预 ,利用3H 胸腺嘧啶核苷掺入法、3H 脯氨酸掺入法和Western杂交测定LFb增殖、胶原合成和Ras蛋白的翻译后修饰。
- trypan blue dye exclusion test 台盼蓝染料排除试验
- Cell toxicity of CL on human endometrial adenocarcinoma cell line JEC(JEC) cells was measured by MTT reduction test and growth curves drawing by trypan blue dye exclusion method. 通过MTT法和台盼蓝拒染法绘制细胞生长曲线研究CL抗人子宫内膜腺癌(JEC)作用。
- Cell Viability is most often defined based on the integrity of the cell membrane and is most commonly measured by observing the exclusion of Trypan blue or other vital dyes. 细胞活性最常用的定义是基于细胞膜的完整性。对细胞浆组分漏入周围培养的测量已被广泛接受为估计非活性细胞数目的一个有效的方法。
- Using trypan bule exclusion test,the cytotoxicity of dir ect lyticfactor(DLF)separted and purified from the venom of Naja atra on human tumor celllines was studied. 用台盼蓝拒染法研究来源于眼镜蛇毒的纯化直接溶解因子(DLF)对培养人肿瘤细胞株的毒性作用。
- Tar showed no cytotoxicity to granulocytes by Evans blue exclusion. Evans蓝排斥试验表明,烟油没有显著的细胞毒性,说明上述抑制现象是粒细胞氧合作用活性,免疫功能受到烟油抑制所致。
- Trypan Blue Dye Exclusion Method 台盼蓝染色排除法
- The dye Trypan Blue has a remarkable biphasic effect on pinocytosis by yolk sal. 染料锥虫蓝对卵黄囊的胞饮作用具有明显的双向作用。
- One of the most successful combinations was trypan blue dye used with a krypton a laser. 实验结果表明,锥虫蓝染料与氪激光器的结合使用是最成功的结合使用之一。
- Cell proliferation was mea-sured by MTT assay and trypan blue excluding experiment. 用MTT和台盼蓝细胞排斥实验检测细胞增殖;
- Trypan blue was used to observe the viability of thymocytes by general light microscope. 采用台盼蓝(Trypan Blue)活细胞拒染法在普通光学显微镜下观察细胞的存活率;
- The expression and function of mdr1 gene were detected by PCR technique,immuno histochemistry (IC) method and DNR exclusion test in vitro and in vivo simultaneously. 同时分别采用PCR技术、免疫组化和柔红霉素排泄试验检测mdr1基因在小鼠体外及体内的表达和功能。
- Trypan blue staining was used to count the alive cells and to draw a cellular growth curve. 台盼蓝染色计数活细胞数,绘制细胞生长曲线;
- Objective To evaluate the efficacy and safety of trypan blue in staining the rabbit's anterior capsule. 摘要目的研究台盼蓝晶状体前囊染色的效果和安全性。
- Methods Twenty-four healthy rabbits were randomly divided into trypan blue and control groups. 方法健康日本大耳白兔24只,分为台盼蓝染色组和空白对照组;
- Results Following the araumatic injury to the cultured neurons, the cell count with trypan blue staining was show... 结论本模型可用于体外研究神经元创伤后原发性和继发性损伤。