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- Crystal structure analysis of drug target proteins complexed with small chemicals. 药物靶标蛋白与小分子化合物的复合物晶体结构研究。
- FBC showed very high affinity to all three essential target proteins in the membrane of K799/WT. 结果表明呋苄青霉素对绿脓杆菌K799/WT的致死性PBPs具有强大的亲和力。
- The recombinant plsmids were transfected into COS-7 cells respectively and to test the expressed target proteins by RT-PCR, ELISA and dot blotting. pcDNA3.;1(+)、pcDNA/Ag85B、pcDNA/MPT64和pcDNA/AM转染COS-7细胞，用RT-PCR、ELISA和斑点印迹的方法鉴定目的基因的表达；
- There are phosphorylation, acetylation, methylation, ubiquitination, SUMOylation and other modifications to modify target proteins. 翻译后修饰包括磷酸化修饰、乙酰化、甲基化、泛素化、类泛素化等不同的修饰。
- These proteins are probably correlated with the causes of disease and can eventually become disease biomarker and treatment target protein. 这些蛋白质与胰腺癌的发生相关，可能成为胰腺癌的分子标志物和药物治疗的靶蛋白。
- The recombinant plasmid pBV220-Arr was construted successfully and the target protein Arresten can express in E. 成功构建Arresten基因重组质粒pBV220-Arr;并可在E.
- The result showed the target protein was successful expressed as a SLH-Ap36 fusion protein. 结果表明，目标蛋白以融合蛋白的形式成功表达。
- After purification, the target protein further renaturation 2 - BMP via degeneration dissolving, load dialysis bag. 纯化后的的目的蛋白进一步复性，BMP-2经变性液溶解，装入透析袋内。
- The reconstructed vector NY-ESO-1-pET-28 could express target protein stably in the soluble fraction of bacterial extract. 构建的原核表达载体NY-ESO-1-pET28a(+)可稳定地可溶性表达NY-ESO-1蛋白;
- Some phages showing low, medium, high specificity to each target protein, lysozyme, insulin and RNase A, had been selected respectively for sequencing. 利用新建立的交替洗脱法，使用其它目标蛋白质对噬菌体展示肽库进行筛选，又获得了对胰岛素和核糖核酸酶A具有高亲和力的噬菌体。
- By expression vector pHHSM002, tpsl gene also expressed efficiently in E. coli BL21(DE3)PlysS ,the target protein amount to 17.8% in cell. 以pHHSM002为表达载体，tps1基因在大肠杆菌BL21中也得到了高效表达，表达的目的蛋白占菌体总蛋白的17.;8%25。
- Pharmacophore hypothesis(PH) is fundamental to de novo design of peptide ligand based on the structure of target protein. 在基于靶蛋白结构的多肽分子设计中，药效假说是从头设计方法的基本前提。
- Other uses of microarrays include the screening of large numbers of molecules for their capacity to bind a particular target protein. 其它微点阵的用途包括从大量分子中筛选出能结合特定靶蛋白的分子。
- The compounds with the fewest side effects are the ones that drop their monkey wrenches selectively, slotting seamlessly into grooves on the surfaces of their target proteins--and leaving other proteins untouched. 那些几乎没有什么副作用的化合物，能选择性地进行攻击并天衣无缝地插入它们的靶蛋白表面的槽内，而使其他蛋白质丝毫不受损。
- The sequences coding two target proteins were cloned into the above recombinant expression plasmids separately and transfected into Cos 7 cells mediated via liposome. The expression products were detected by Western blot and co immunoprecipitation. 将两种候选蛋白的编码序列分别克隆于上述融合载体,转染Cos?7细胞后的表达产物,可利用抗HA或myc的mAb进行Western?blot和免疫共沉淀。
- SUMO protease can cut SUMO fusion protein expressed by fusion expression system without any amino acid residues left on target protein thus become a hot topic in this field. SUMO蛋白酶对SUMO融合表达系统表达的重组蛋白进行切割时没有多余氨基酸残留，因此成为蛋白切割工具的热点。
- Using different density urea to isolate the inclusion body, then purify the target protein through the Ni-NTA resin, analyzed with SDS-PAGE found the purity attained over 90%. 用尿素梯度分离包涵体，确定1 M尿素洗涤包涵体为分离MP-GFP的最优条件。经Ni-NTA树脂纯化目标蛋白，SDS-PAGE电泳分析其纯度达到90%25以上。
- The 14-3-3 proteins interact with targeting proteins or two different domains in one targeting protein by phosphoserine /phosphothreonine motifs. 7种不同亚型的高度保守的14-3-3蛋白与人类细胞中多种不同的信号通路有密切的关系。
- The torpedo homed in on its target. 鱼雷射向目标。
