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- To construct subtractive cDNA library from the bone of apical facets in AIS patient with suppression subtractive hybridization (SSH) technique. 通过构建AIS患者脊柱关节突骨组织抑制消减cDNA文库，寻找AIS患者顶椎凸、凹侧关节突骨组织的差异表达基因，探讨AIS可能的病因。
- Dominating methods in wheat gene cloning include homology-based cloning, map-based cloning, suppression subtractive hybridization, DDRT-PCR etc. 目前，小麦基因的克隆方法主要有同源克隆、图位克隆、差减杂交、差异显示等。
- BACKGROUND & OBJECTIVE: Subtractive hybridization technology is a common method to screen and clone differentially expressed genes. 背景与目的:消减杂交技术是一种寻找和克隆差异基因的方法。
- Methods The gene was cloned by improved PCR-based subtractive hybridization from apoptotic prostate cancer cell line DU-145 cells induced by ATRA. 方法 应用全反式维甲酸诱导前列腺癌细胞系DU-145细胞凋亡，采用基于PCR的改良消减杂交技术克隆与凋亡相关的基因。
- Suppression subtractive hybridization (SSH), which is feasible and highly specific, is one of the most efficient methods for cloning differentially expressed genes. 摘要抑制性消减杂交是一种简便易行、特异性高的高效分离差异表达基因的方法。
- Suppression subtractive hybridization(SSH),which is feasible and highly specific,is one of the most efficient methods for cloning differentially expressed genes. 抑制性消减杂交是一种简便易行、特异性高的高效分离差异表达基因的方法。
- Acute promyelocytic leukemia, Antimonials, Arsenicals, All-trans retinoic acid, PCR, Subtractive hybridization, Batch gene cloning, New therapy for APL. 01急性早幼粒细胞白血病,锑剂,砷剂,全反式维甲酸,PCR,消减杂交,基因克隆,新疗法
- To identify the genes controlling water stress response in maize (Zea mays) seedlings, suppression subtractive hybridization (SSH) was performed using mixed cDNAs pre. 水分胁迫严重影响植物生长及作物生产，它是主要的非生物胁迫因子之一。
- The products of second subtractive hybridization were cloned into the vector of pUC57/T to create a subtractive library. 510 Clones were selected from it and were identified by PCR. 将经过两次消减杂交及PCR扩增后的产物经“A一T clone”克隆到pUC57/T载体，进而转染大肠杆菌获得消减文库，共挑选出510个克隆，经PCR鉴定有特异性扩增带且扩增带分子量在200一700bp的克隆有433个，阳性率高达
- Objective: To construct a subtractive cDNA library of genes transactivated by homo sapiens NS5A-TP4 using suppression subtractive hybridization (SSH) technique and to clone genes associated with its transactivating function. 目的:应用抑制性消减杂交(SSH)技术构建人类新基因NS5A-TP4反式激活基因差异表达的cDNA消减文库,克隆NS5A-TP4反式激活相关基因,了解该基因的可能生物学功能。
- Four full length cDNAs of hatching enzyme genes were cloned from gynogenetic gibel carp ( Carassius auratus gibelio) by suppression subtractive hybridization, SMART cDNA synthesis and RACE PCR. 用抑制性差减杂交结合SMARTcDNA合成和RACE PCR技术 ,克隆得到雌核发育银鲫 (Carassiusauratusgibelio) 4种孵化酶基因的全长cDNA。
- There are several methods widely applied, including suppression subtractive hybridization (SSH), serial analysis of gene expression (SAGE), cDNA microarrays, silicon cloning and et al. 表达基因分析方法不断完善，包括抑制消减杂交技术，基因表达序列分析，基因表达谱芯片，电子克隆等。
- The general principle, procedure, advantages and shortcomings of the suppression subtractive hybridization (SSH) were summarized. The recent developments of SSH in gene cloning were also described. 摘要简要概述了抑制性消减杂交技术的基本原理、基本过程、优越性、主要缺陷及其在基因克隆方面的应用进展。
- solid-phase subtractive hybridization 固相消减杂交
- suppression subtractive hybridization (SSH) 抑制消减杂交
- genomic DNA subtractive hybridization 基因组DNA递减杂交
- suppressed subtractive hybridization 差异表达
- suppressing subtractive hybridization 抑制性消减杂交
- suppression subtractive hybridization ( SSH) 抑制差减杂交
- suppression subtractive hybridization (SSH) library 抑制性消减文库（SSH）
