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- RT-PCR showed that E1A gene had a stable transfection in Hela cells and obviously down-regulated Her-2 expression. RT-PCR结果显示:E1A基因在Hela细胞中能稳定表达,E1A基因的转染明显降低了Her-2基因在Hela细胞中的表达水平;
- RT-PCR demonstrated that E1A gene had a stable transfection in Hela cells and obviously down-regulated HER-2/neu expression. RT-PCR结果显示:E1A基因在宫颈癌细胞中能稳定表达并且显著降低HER-2/neu在宫颈癌细胞中的表达水平。
- Stable transfection of FP248 gene to the SMMC-7721 cell line demonstrated a promoting effect on the proliferation of SMMC-7721 cell line in vitro and in vivo. 由此可见，FP248对SMMC-7721细胞的生长具有促进作用，以上的实验给了我们一致性的结果，由此我们可以推断FP248在肝癌的发生发展中具有重要的作用。
- The positive cell clones had been obtained after selected by G418. The stable transfection and expression of AIRE in HeLa cells were determined by the fluorescence microscope, FACS and Western blotting. 继续培养 4周 ,用荧光显微镜观察、流式细胞仪 ( FACS)检测和免疫印迹法检测 AIRE基因在 He La细胞中的稳定表达情况。
- Abstract：Objective To investigate the effects of exogenous MTH gene stably transfection on growth of lung adenocarcinoma cell A549 in vitro. 目的研究外源性人金属硫蛋白H（MTH）基因稳定转染对肺腺癌细胞A549生长的影响及其机制。
- The chemotaxis and calcium mobil i zation assay were used for examining bioactivities of the cells stably transfect ed with CCR4. 通过基因转染技术获得稳定转染细胞株，利用趋化实验、钙流实验证实稳定转染细胞株能有效表达CCR4并具有生物功能。
- Keywords hydatidiform mole;F10 gene;stable transfection; 葡萄胎;F10基因;稳定转染;
- The only successful study of ribozymes against HBV in cells was shown in a stable transfectant that endogenously produced hairpin ribozymes. 唯一在细胞内核酶成功抑制HBV的研究是转入内源性稳定表达系统来表达发夹样核酶。
- Keywords IBP;siRNA;Stable transfection;Apoptosis;Immunological homeostasis; 稳定转染;细胞凋亡;免疫自稳;
- The evils ensue from lack of a stable government. 这些弊病是由于缺乏一个稳定的政府而引起的。
- Objective To construct HBVx-GFP eukaryotic expression plasmid and establish stable transfected HepG2 cell line expressing HBVx-GFP fusion protein for exploring the biological function of HBVx and its roles in carcinogenesis of hepatocellular carcinoma. 目的构建绿色荧光蛋白(GFP)与人乙型肝炎病毒(HBV)X基因的重组表达载体,建立稳定表达HBVX蛋白(HBx)与GFP融合蛋白的HepG2细胞系,以进一步研究HBx的生物学功能及其在肝癌发生中的作用。
- The recombinant protein from the extracts of stable transfected TG1 was purified by one step affinity chromatography. The molecular mass of the product, with RGS His6 tag, is 32 ku. 将该基因重组到表达型质粒pQE30中 ;表达的His6Tag Ag85B融合蛋白分子质量约 32ku .
- The horse in the stable has broken out. 马厩里的那匹马跑了。
- He is a man of stable character. 他是一个稳重可靠的人。
- The black horse is one of Mr. King's stable. 那匹黑马是金先生的赛马之一。
- That country is politically stable. 该国政治上稳定。
- He has a whole stable of informers working for him. 他有一群告密者为他效劳。
- There were six stalls for horses in the stable. 马厩里有六个栏。
- A stable, corrosion-resistant zirconium alloy. 锆合金一种稳定而防腐蚀的锆合金
- Methods: PC12 cells were stably transfected with pEGFP-C2/5-LOX (GFP/5-LOX) or pEGFP-C2 vectors (control). 方法:将带有5-LOX基因的pEGFP-C2质粒及pEGFP-C2质粒(对照),稳定转染至PC12细胞;
