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- The established specific PCR assay should provide a practical and rapid method for the identification and differentiation of Eimeria species in mixed infections. 用本项研究所建立的特异PCR方法对这些混合感染的球虫种类进行鉴定具有一定的实用价值。
- specific PCR assay 特异PCR
- Moreover,this PCR protocol can detect Cff sufficiently in 100 pg template DNA. It was demonstrated to be a highly sensitive,specific and rapid PCR assay for the detection of Cff. 该检测方法特异性强、灵敏度高 ,在 10 0pg的基因组DNA浓度下仍能检测到很强的条带
- Objective To establish a PCR assay for the detection of Helicobacter pylori. 目的建立一种PCR方法检测幽门螺杆菌(Helicobacter pylori,Hp)感染。
- Objective To establish PCR assay of Porcine Parvovirus detection in Trypsin. 目的建立原材料胰蛋白酶中猪细小病毒的PCR检测方法。
- Development and application of multiplex PCR assay for the detection of Campylobacter spp. 弯曲菌多重PCR检测方法的建立及其初步应用。
- PCR assay used to detect the conserved toxoplasmic genes also showed positive result. 应用PCR方法检测弓形虫保守基因，结果为阳性。
- It is indicated by PCR assay that the aim gene have been integra ted into maize genome. PCR检测证明，目的基因已整合到玉米基因组中。
- The methylation specific PCR (MSP) and bisulfite DNA sequencing were performed to examine the methylation status of SLIT2 gene promoter. 亚硫酸氢钠处理DNA,用MSP和测序法检测SLIT2启动子甲基化状态。
- Methods Detecting SFGR DNA sequence fragment in spleen of rodents and ticks by PCR assay. 方法用PCR法检测鼠类脾脏和蜱类中的斑点热群立克次体DNA序列片段。
- The sensitive and specific PCR method for detection E. granulosus from dog faeces was preliminarily developed. 初步建立了灵敏、特异的检测家犬粪便中细粒棘球绦虫的PCR方法。
- Method Several pairs of specific PCR primers were designe d according to the conserved region of HDV genome. 方法针对HDV基因保守区域设计多对PCR引物。用芯片点样仪将PCR产物点到玻片上制成基因芯片。
- PCR assay and Southern blot analysis showed that CMO gene was integrated successfully into the genome of transformed plant. PCR及Southern检测证明CMO基因已整合进抗性植株基因组;
- The mean HBV DNA level decreased 5.9 lg copies/ml(by PCR assay)from baseline in ETV group versus 4.3 lg copies/ml in LVD group(P<0.0001). HBVDNA的载量(PCR定量);ETV组平均下降5.;9lg拷贝/ml;LVD组平均下降4
- Conclusion The sensitive and specific PCR method for detection E. granulosus from dog faeces was preliminarily developed. 结论初步建立了灵敏、特异的检测家犬粪便中细粒棘球绦虫的PCR方法。
- In addition, there were 2 negative samples in PPD test, while they were positive in nested PCR assay and also in FQ-PCR assay. 在对2份PPD检测阴性而巢式PCR检测阳性的临床样本的检测结果也为阳性。
- Methods The methylation status of CpG island of AR gene promoter in 3 leukemic cell lines and 15 leukemic bone marrow cells was detected by methylation specific PCR(MSP). 方法应用甲基化特异性PCR技术(methylation specific PCR,MSP)检测3种白血病细胞株及15例患者骨髓标本雄激素受体基因甲基化状态。
- Conclusion The pandemic group-specific multiplex PCR assay can fast identify pandemic vibrio parahaemolyticus and providing a methodology for searching epidemic pathogen. 结论群特异多重PCR法能快速鉴定当前副溶血性弧菌流行群，有利于流行病学溯源等。
- Methods The specific PCR primers were designed and synthesized to amplify the DNA fragments enconding preS1/S2/S antigen gene from the plasmid pHBV adr by PCR method. 方法设计合成2对寡核苷酸引物,以adr亚型HBV质粒pHBV DNA为模板,采用PCR法分别扩增HBV大分子表面蛋白基因(preS1/S2/S基因)片段;
- The real-time PCR assay was evaluated using 60 B. cereus group strains and 28 others.The assay was also used to construct calibration curves for different food matrices and feces. 共使用60株仙人掌杆菌群菌株以及28株其他菌株进行即时聚合酶链反应方法之评估，并应用于不同之食品基质及粪便检体。
