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- Single strand conformation polymorphism (SSCP) essay and sequence analysis of the PCR product were used to ascertain the gene mutation. 方法应用PCR及单链构象多态性(SSCP)分析技术结合基因序列测定方法确定突变类型。
- Exon 6, 13, 15, 17 of RET proto-oncogene were amplified by polymerase chain reaction (PCR) and analyzed by single strand conformation polymorphism (SSCP). 聚合酶链反应(PCR)扩增RET基因第6、13、15、17外显子;单链构象多态(SSCP)分析上述外显子是否有突变。
- Methods: (1) Using polymerase chain reaction(PCR) combined with single strand conformation polymorphism (SSCP) method and DNA sequencing to detect the point mutation of keratin 14 in a pedigree of DM-EBS. 采用多聚酶链反应(PCR)、单链构象多态性(SSCP)分析、DNA直接测序方法,对DM-EBS一家系角蛋白14(K14)进行基因分析。
- An A/C polymorphism was identified at nucleotide-11 from the acceptor site of IVS3 of the phenylalanine hydroxylase gene. This polymorphism can be detected easily by means of single strand conformation polymorphism analysis. 在苯丙氨酸羟化酶基因IVS3的3'端-11位有一个A/C多态性位点,应用SSCP分析可进行简便检测。
- METHODS: Using the method of polymerase chain reaction single strand conformation polymorphism (PCR-SSCP), the point mutation of the ABCB4 gene exon 6 and exon 14 in 31 women with ICP were detected. 方法:采用聚合酶链反应单链构象多态性(PCR-SSCP)方法对31例妊娠期肝内胆汁淤积症患者进行ABCB4基因外显子6和外显子14的点突变筛查。
- single strand conformation polymorphism 单链构型多态性
- single strand conformation polymorphism(SSCP) 单链构象多态性
- PCR single strand conformation polymorphism PCR-SSCP
- single strand conformation polymorphism analysis 单链构象多态性分析
- single strand conformation polymorphisms 单链构象多态性
- Study on Principles of DNA Single Strand Conformation Polymorphism DNA单链构象多态性原理初探
- methylation sensitive single strand conformation analysis MSSSCA
- methylation sensitive single strand conformation analysis(MSSSCA) 甲基化敏感单链构象分析
- PCR single strand conformation polymorphism(PCR-SSCP) 聚合酶链反应单链构象多态[可用于研究基因突变和基因多态性]
- single strand conformation polymorphism(SSCP) analysis SSCP分析
- Expression of Fas was analyzed by a FACScan flow cytometry. Transcription and mutation of Fas gene were detected by RT PCR and single stranded conformation polymorphism(SSCP) analysis. 用流式细胞仪检测细胞表面Fas的表达 ,用RT PCR结合单链多态构象性分析 (SSCP) ,探讨Fas基因的转录及可能存在的Fas基因突变。
- polymerase chain reaction single strand conformation polymorphismanalysis PCR-SSCP
- A single strand lives for up to seven years. 一根头发的寿命可长达7年。
- An automated fluorescent single strand conformation polymorphism technique for high throughput mutation screening 适用于规模性基因突变扫描的荧光标记单链构象长度多态性检测方法分析
- Single Strand Conformation Polymorphism Analysis of Polymerase Chain Reaction Products, PCR-SSCP 聚合酶链反应-单链构象多态性分析