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- The Tat signal peptide detecting vector of PGA were constructed. 构建了Tat信号肽检测载体。
- We add the 5'AOX1 primer and 3'AOX1 primer amplify pPIC9K and alpha-factor signal peptide sequence was obtained. 考虑到克隆方便,在序列5’端和3’端分别引入BamHI和EcoRI 位点,并在两个酶切位点的外侧分别加上保护碱基。
- Once across the membrane, the signal peptide is cleaved by the signal peptidase and the process protein is released. 信号肽是疏水的，与膜上结合的蛋白接近，拉着后面的亲水多肽跨膜，一旦跨过膜，即被信号肽酶切除，释放出加工的蛋白。
- The location of the signal peptide of this peptide chain is 1-26, the breaking point is between 26 and 27 (GAA-AF). 应用神经网络算法和隐马氏模型分析结果显示该肽链信号肽位置为1-26，断裂点位置是26-27（GAA-AF）。
- The extramembrane region consists of 758 aminoacids, including signal peptide containing 31 amino acids at N-terminus and seven Ig-like domains. 胞外区由758个氨基酸组成，包括N端31个氨基酸的信号肽和七个类似免疫球蛋白(Ig)的结构域。
- There were no signal peptide and N-glycosylation sites, while had 4 conservative Cys amino acid in the deduced amino acid sequence. 在推导的猫IL-18氨基酸序列中,无信号肽序列和潜在的N-联糖基化位点,但存在4个Cys残基。
- The signal peptide at the N- termination can lead the protein to the periplasm.Six histidines at the C- termination will let the purification easy. 在质粒pET-20b(+)中，重组的蛋白会在N-末端带有一个信号肽序列，它可以使重组蛋白分泌到细胞的周质空间处，而其在C-末端还有带有6个组氨酸的标签，这样就使得重组蛋白的分离纯化更加的方便，简单。
- Proteins secreted by one step into the periplasm or through Bayer's junctions to the extracellular environment have a signal peptide. 蛋白可直接分泌到周质中，或通过Bayer接点分泌到胞外环境中的蛋白有一个信号肽。
- In this paper, signal peptide was added before the N-terminus of CEMA with the intention to reduce its toxic to plant cells while maintaining the strong antibacterial activity. 本文通过在CEMA的N端添加信号肽，对其进行改造，以探索不降低抑菌作用的同时，降低其对植物细胞毒害作用的可能性。
- GliTd-3 and GliTd-4 were both 861bp, and their deduced amino acid sequences were both 267 residues with a 19-residue putative signal peptide (absent from mature proteins). 而GliTd-1和GliTd-2编码区分别长821bp和859bp，由于编码区内存在提前终止密码子和插入/缺失引起的移码突变，推测其为不表达的假基因。
- With the signal peptide analysis software of the TMHMM2.0(http://genome.cbs.dtu.dk/services /TMHMM-2.0), the preceding peptide of Vip3A-WB5 was a transmembrane peptide. 通过TMHMM2.;0(http://genome
- Fibroblast growth factor（FGF-1）lacks amino-terminal signal peptide, so it can’t release through the classical endoplasmic reticulum（ER）-Golgi pathway. 酸性成纤维生长因子-1（FGF-1）由于氨基端缺乏信号肽序列，不能通过经典的内质网-高尔基体途径释放。
- With common molecular biology technique,we cloned SCF gene including signal peptide coding sequence coded signal peptide and constructed its expression plasmid. 采用常规的分子生物学技术,从小鼠骨髓细胞中克隆了含信号肽序列的SCF,并构建了表达载体。
- This article reviews signal peptide sequence about its structural feature,the common used signal peptide and the construction of vector with high secretory. 本文将从信号肽结构特征、常用的信号肽序列以及构建高分泌型载体等方面进行综述。
- Signal peptide was found on former 20 amino acids of both two coding regions of protein, one strong and one feeble transmembrane helices were also found. 2. 并在两种酶蛋白编码区的前20个氨基酸发现信号肽序列，通过跨膜区预测，均发现一个较强的跨膜信号和一个较弱的跨膜信号。
- The fusion DNA fragment of the lactococcal extracellular protease USP45 signal peptide, passenger protein and SBC were expressed in Lactococcus lactis and Lactobacillus paracasei. 融合乳酸菌胞外蛋白酶USP45讯息胜肽、乘载蛋白及表层蛋白质静默基因C端123个胺基酸，表现于副乾酪乳杆菌或乳酸链球菌。
- Obtaining of SPCEMA(signal peptide modified CEMA)SPCEMA(187bp) was amplified with two long complementary primers (P2 and P3) and two primers (Pl and P4) containing restriction enzyme recognition site. 以两条部分重叠长链引物P_2和P_3延伸产物为模板，P_1和P_4为正、反引物进行PCR扩增，获得了改造的抗菌肽基因SPCEMA(187bp)。
- The signal peptide, KDEL and E tag sequence was successfully introduced into 9.1C3 ScFv, and the intrabody gene was inserted into eukaryotic expression vector which can be expressed in COS7 cells. DNA序列测定证明 ,通过PCR成功地为 9 1C3ScFv引入了内质网定位所需的信号肽、KDEL及E tag序列 ,成功地构建了胞内抗体真核表达载体 ,通过胞内染色方法和Westernblot证明胞内抗体在COS7中得到表达。
- Sequence analysis of Gal-4 eDNA showed that the cDNA of Gal-4 gene con- sists of 321 bp,encoding Gal 4 polypeptide of 63 amino acids,which comprised of signal peptide with 20 amino acids at N'terminus,and mature peptide with 38 amino acids. 序列分析结果表明,获得的广西黄鸡Gal-4基因大小为321 bp,推导的Gal-4由63个氨基酸组成,其中N端20个氨基酸为信号肽,C端38个氨基酸组成Gal-4的成熟肽。
- The SJCWL06 gene is 861 bp in whole length, has an open reading frame (ORF) of 327 bp encoding 109 amino acids with a signal peptide, an ABC-ATPase domain, several phosphorylation sites and the highest similarity of SMC3 protein of Apis mellifera (84%). 该基因长861bp,有一个完整的327bp的开放读框,编码109个氨基酸,其编码蛋白有一个信号肽,多个磷酸化位点和一个ABC-三磷酸腺苷酶的结构域,与蜜蜂(Apis mel-lifera)SMC3编码的mRNA基因序列同源性最高(84%25)。
