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- Compared with shaking flask cultivation, Tobramycin fermentation in 5L auto fermenter has been studied systematically. 分析和对比了不同溶氧情况下妥布拉霉素菌株在摇瓶和5L全自动发酵罐中发酵过程的特征。
- Its fermentation media in shaking flask were optimized. Average yield of SSG came to 12. 03g/L,percentage yield bassed on soluble starch was 40. 1%. 本文通过单因子试验和正交试验对其摇瓶发酵条件进行了优选,使SSG的产率达到12.;03g/L,碳源转化率达40
- Addition of propanol during erythromycin fermentation with in shake flask improved the titer by 49. 在摇瓶培养过程中,发酵初期加入正丙醇可使红霉素的效价提高49。
- Results: shaking flask titer productivity of 259# strain obtained by selective cultivation was higher than parent strain. 结果:选育得到的259%23菌株摇瓶效价比出发菌株提高,传代三代后仍稳定。
- The highly yielding strain J-12 was screened from Xanthomonas campestris by plate and shake flask culture experiments . 通过平板初筛、摇瓶发酵复筛,得到高产菌株J-12。在此基础上,采用单因素实验探讨了碳源、无机盐、柠檬酸和接种量对黄原胶产率和发酵液粘度的影响。
- Potassium release from soil and yield increase of crops by strain NBT of silicate bacterium were studied by pot,field and shake flask experiments. 通过盆栽试验、田间试验以及摇瓶试验研究了硅酸盐细菌NBT菌株的解钾作用以及对作物的增产效应。
- The fermentation culture medium and shaking flask fermentation conditions of glutathione have been researched by using orthogonal experiments with Saccharomyces cerevisiae. 通过正交试验,研究了啤酒酵母摇瓶发酵生产谷胱甘肽的培养基的组成以及发酵培养条件。
- Methods: In shaking flask test, We used Laurate alcohol ester phenylacetate as precursor and supplementary material instead of sodium phenylacetate used in former process. 方法:在摇瓶试验中用苯己酸月桂醇酯代替原工艺所用苯乙酸钠作为前体及补料用,在相同条件下与原工艺对照比较,进行考察研究。
- Production f nourishing healthful vinegar by shake flask culture with the raw material of Carica papaye from Changyang County, Hubei was studied. 以湖北长阳县的木瓜为原料,采用液体摇瓶发酵法,制备营养丰富的保健醋。
- The effects of inorganic and organic nitrogen sources on synthesis and secretion of gentamicin in shake flask fermetation were studied. 研究了无机与有机氮源对庆大霉素合成与分泌的影响。
- The production of erythromycin in the shake flask is apparently influenced by adding different kinds of organic nitrogen source. 红色糖多孢菌的摇瓶培养基中采用不同有机氮源时红霉素的效价明显不同。
- Shaking flask and pot experiment were carried out to study the dissolution of feldspar and growth promoting of the plants by the strain NBT of silicate bacterium. 摘要 在摇瓶和土壤耗竭条件下研究了硅酸盐细菌NBT菌株的解钾作用以及对作物生长的促进作用。
- The optimum culture conditions of genetically engineered Pichia pastoris in shake flask cultivation and in fed batch fermentation were investigated respectively. 在摇瓶条件下对基因工程菌 Pichia pastoris的发酵条件进行了实验 ,并根据摇瓶发酵的优化结果进行了补料分批高密度发酵。
- Shake flask culture was used and the yield of polysaccharide was taken as main index to optimize the culture medium of submerged fermentation for Ganoderma lucidum G22. 采用摇瓶液体发酵的方法,以灵芝多糖为指标,对赤芝G22菌株液体发酵培养基进行了研究。
- Influences of carbon and nitrogen sources on Pseudomonas putida S1 dry weight and on the activity of intracellular trehalose synthase,were studied in shaking flask scaled Fermentation. 采用摇瓶发酵法研究了不同碳源、氮源对恶臭假单胞菌S1干重和胞内海藻糖合成酶酶活力的影响。
- Methods: To mutate breeding for strain by chemical mutagen.Its producing resistant ability and propagating stability of strain obtained by screening was observed by shaking flask test. 方法:用化学诱变剂对菌种进行诱变育种,筛选得到的菌株通过摇瓶试验考察其产抗能力与传代稳定性。
- The experiment for optimization of the Flavomycin fermentation medium was carried out with the design of the rotation-regression-orthogonal combination under the shaking flask condition. 在摇瓶条件下,采用二次回归正交旋转组合设计,优化黄霉素发酵培养基各组份的配比。
- Strain A was selected as ADY production yeast, its suitable culture temperature was 32 C, specific growth rate was 0.2481/h in malt extract, trehaloase accumulation could reach 8.59% in shake flask. 选定菌株A作为葡萄酒活性干酵母生产菌株,其最适培养温度为32℃,在麦芽汁培养基中的最大比生长速率为0.;2481/h,在摇瓶培养条件下,海藻糖的积累量可达细胞干重的8
- The HPLC method with a good result of separation has been developed for the determination of gentamicin intermediate ingredients, inclusive of shake flask, fermentation broth and desorption solution. 应用HPLC法测定庆大霉素中间体,包括摇瓶、发酵液、解吸液等的组份分析,分离效果良好,具有使用价值。
- The new chairman will shake up the company. 新任董事长将改组这家公司。