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- Methods The expression of MDR1 gene in 77 cases of AL patients and 10 cases of control group was detected with semi quantitative reverse transcription polymerase chain reaction (RT PCR). 方法 采用半定量逆转录聚合酶链反应（ R T? P C R），对77 例 A L病人及 10 例正常人 M D R1 基因表达进行检测。
- The mRNA levels of this gene were determined in SCLC cell line SH77 ,big cell lung cancer cell line H460,lung adenocarcinoma cell line SPC A 1 and SPC A 1/CDDP using semi quantitative RT PCR assay. 应用半定量RT-PCR方法检验小细胞肺癌SH77、大细胞肺癌H460、肺腺癌SPC-A-1和SPC-A-1/cDDP等细胞株中该基因mRNA的表达。
- RT PCR was performed for APN gene expression in liver tissue. (2 )APN表达 :根据兔APN基因cDNA序列设计引物 ,提取肝总RNA ,利用RT PCR方法 ,检测肝APNmRNA水平的变化。
- Methods Plasma ETA was measured quantitively by RT PCR. 方法 应用 RT-PCR技术检测外周血循环中 ETA基因表达水平。
- Angiotensin receptor (ATR) mRNA expression of VSMC was measured by RT PCR. RT PCR检测VSMC血管紧张素受体 (ATR)mRNA表达。
- Expression of the M 2-1 gene was examined by RT PCR and Western blot. 重组真核质粒PXJ 4 1/M2 -1转染肺腺癌PAa细胞 ,应用RT PCR、Westernblot方法检测表达。
- The expression of cyclin D1 and CDK4 were detected by RT PCR method. RT PCR检测细胞周期素D1(cyclinD1)、细胞周期蛋白依赖激酶 4 (CDK4 )mR NA的表达。
- RT PCR for EWS FLI 1 is sensitive enough to detect minimal residual Ewing's sarcoma cells in the bone marrow. 以EWS FLI 1融合基因为标志的反向PCR测定骨髓Ewing肉瘤细胞的灵敏度为 10 -6,它是测定骨髓Ewing肉瘤细胞高度敏感的方法 ;
- The positive rate of MDR1 mRNA was 70.3% with improved RT PCR in 37 among 49 cases of ovarian carcinomas. 有37 例卵巢癌用改进的RT?PCR 检测MDR1 基因m RNA 表达的阳性率为70?3 %25 。
- B7 1(CD80) cDNA was cloned by RT PCR from human B lymphma Raji cell line and confirmed by DNA sequencing. 应用RT－PCR技术从人B淋巴瘤细胞系Raji中克隆到B7－1（CD80）cDNA，并经测序证实。
- Methods B7 expresion was studied by means of RT PCR and immunohistochemistry (IH) in 45 tumors of the urinary system. 方法应用逆转录?聚合酶链反应（RT?PCR）和免疫组织化学方法，检测45例泌尿系肿瘤B7基因在mRNA和蛋白水平的表达情况。
- Methods HGV RNA in plasma of 84 IVDUs was detected by reverse transcription polymerase chain reaction (RT PCR). 方法采用逆转录聚合酶链反应（RT－PCR）检测84例静脉毒瘾者血浆标本。
- Methods RT PCR was used to explore IL 1Ra mRNA expression in middle cerebral artery occlusion (MCAO) rat model. 方法 采用线栓法制备SD大鼠局灶性大脑中动脉阻塞 (MCAO)动物模型 ,应用RT PCR方法观察脑缺血再灌注对大鼠IL 1RamRNA表达的影响。
- After the DC were transfected with pCA13/EWS FLI1,the EWS FLI1 mRNA was easily detected by RT PCR. pCA13/EWSFLI1转染DC后,RTPCR检测DC中有EWSFLI1mRNA的表达。
- Methods: The expression of B7 in 22 cases of renal cell carcinoma was studied by RT PCR and immunochemical methods. 方法：本文应用RT?PCR和免疫组织化学技术检测了22例肾细胞癌组织B7的表达。
- It can be measured the expression of c jun and c fos by using the methods of RT PCR and agarose gel electrophoresis. 应用RT PCR和琼脂糖凝胶电泳方法测定c jun和c fos基因的表达。
- Total RNA was extracted from PC12 cells. The TRX cDNA obtained by RT PCR was cloned into PUC18vector. 从PC1 2细胞中提取总RNA ,经逆转录PCR(RT PCR)扩增出硫氧还蛋白cDNA并克隆到PUC1 8质粒上。
- Methods Using HE stains,Niles stains,transmission electron microscope,immunohistochemistry and RT PCR technique etc. 方法采用HE染色、尼氏染色、透射电镜、免疫组化及RT-PCR技术。
- Methods The expressions of AQP1-9 in various organs of male reproductive tract of mouse was determined using RT PCR. 方法 :应用 RT- PCR技术检测 AQP1 - 9在雄性小鼠生殖系统不同器官的表达。
- RT PCR analysis showed that the maternal mRNAs of the gibel carp M3 CK gene was detected in mature eggs and early stage embryos. RT PCR表明 ,银鲫M3 CK基因在成熟卵母细胞和胚胎发育早期可检测到少量的转录产物 ,在胚胎发育期间从肌肉效应期开始转录 ,并一直持续表达。
