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- revert dot blotting RDB
- revert dot blotting(RDB) 逆向点杂交技术
- Effect of labeling was detected by dot blot hybridization. 点杂交方法检测探针标记效果。
- Apply the PCRDIG probe to quickly detect the SRY gene by DNA dot blotting. PCR-DIG探针斑点杂交法快速检测SRY基因
- Methods Polymerase chain reaction(PCR) connected with reverse dot blot(RDB) were performed. 方法采用多聚酶链反应(PCR)结合反向斑点杂交(RDB)技术。
- ET?1 and NOS mRNA from the gastric mucosa of the three groups were measured quantitatively by Dot blot technique. 采用Dotblot杂交技术定量研究3组胃粘膜ET?1、NOSmRNA表达。
- It was proved that the E2 genes were integrated stably into chromosome of P.Pastoris by Dot blot and DNA sequencing. Pastotis进行整合,经G418筛选得到25个高拷贝转化子,经DNA斑点试验和DNA测序证明外源基因E2稳定地整合到P.;Pastoris染色体中。
- Using the 900bp fragment labeled by DIG as probe, dot blotting analysis demonstrated Sox9 was obviously present in the allotetraploid fish genome. 用地高辛标记的900bp片段做探针,斑点印迹结果显示,Sox9基因明显存在于四倍体鱼基因组中。
- The recombinant plsmids were transfected into COS-7 cells respectively and to test the expressed target proteins by RT-PCR, ELISA and dot blotting. pcDNA3.;1(+)、pcDNA/Ag85B、pcDNA/MPT64和pcDNA/AM转染COS-7细胞,用RT-PCR、ELISA和斑点印迹的方法鉴定目的基因的表达;
- Besides, the gene expression of c-myc, wtp53, p16 and EGFR was detected by RNA dot blot. 应用完整细胞原位斑点印迹杂交技术检测c-myc、野生型p53(wtp53)、p16和EGFR的基因表达;
- Denatured-reduced forms of MBP-fusion proteins in immunoblotting, dot blot and ELISA.General. 特异性 Monoclonal Anti-GFP recognizes wild type; recombinant; and enhanced form of GFP.
- Objective: To study the clinical value of detecting mycobacterium tuberculosis by dot blot hybridization. 摘要目的:探讨斑点杂交法检测结核分枝杆菌的临床应用价值。
- From recombinant plasmid pGEMTH2 prepared cRNA probe was identified by dot blot hybridization. 使用斑点杂交证实我们制备的探针是敏感而可靠的。
- Rearrangement and amplificarion of erbB,sis,myc and fos in brain tumors are studied with DNA dot blot and Southern blot analysis. 本文用 DNA 斑点杂交法和 Southen 印迹法对脑瘤中 erbB、sis、myc 和 fos 这四种癌基因的扩增和重排进行了研究。
- The results of Dot Blotting and Western Blotting showed that the expressed hBMP4 and hBMP7 were identified separately by anti-BMP4 antibody and anti-BMP7 antibody. 经斑点印迹和蛋白质印迹证实了表达产物含有hBMP4与hBMP7,均具有良好的抗原性和特异性。
- The 16S rRNA PCR-membrane reverse dot blot hybridization technique showed that the sensitivity was 92.49%, and the specificity was 100%. PCR-膜反向斑点杂交技术鉴定分枝杆菌菌种的灵敏度为92.;49%25;特异度为100%25。
- After random labeling with DIG, RNA dot blot was proceed to test the expression of this gene under different growth condition. 用地高辛随机引物标记试剂盒对该片段进行标记后,对不同水分和硫营养条件下提取的小麦根系总RNA 进行斑点杂交。
- Dot blot and southern blot analyses suggested that gfp gene is integrated into the genome of transgenic plants of Nicotiana tabacum, Pentunia genome. 通过对有绿色荧光的烟草、矮牵牛进行dot blot、southern blot分析,都获得阳性杂交结果,证实gfp基因已整合到植物基因组中并与绿色荧光观察结果一致。
- Methods:Polymerase chain reaction in combination with dot blot hybridization of allele specific oligonucleotide(PCR/ASO) probes was used. 方法:采用聚合酶链反应结合等位基因特异的寡核苷酸探针杂交(PCR/ASO)技术。
