您要查找的是不是：
- repeat amplification protcol 重复扩增
- Telomerase activity of GBC SD cells was examined by telomeric repeat amplification protocol (TRAP). TRAP法检测端粒酶活性,流式细胞仪检测细胞周期及凋亡情况,电镜观察细胞微观形态变化。
- Methods:The telomeric repeat amplification protocol(TRAP)combined with densitometry and clinical examination were performed in leukemia patients. 方法：端粒重复扩增法结合吸光度测定及临床病例分析。
- Telomerase activity was determined by the telomeric repeat amplification protocol enzyme-linked immunoassay (TRAP -ELISA). 应用TRAP-ELISA法测定肾上腺肿瘤中的端粒酶活性。
- Methods The telomeric repeat amplification protocol (TRAP)combined with semiquantitative analysis and clinical obsevation of arsenic trioxide treatment in leukemic patients. 方法端粒重复扩增法结合光密度测定和临床治疗观察。
- The telomerase activity in mouse oocyte and preimplantation embryo was examined using the polymerase chain reaction-based on telomeric repeat amplification protocol (TRAP). 摘要为了解小鼠早期发育过程中端粒酶的活性变化，本研究利用端粒重复序列扩增法(TRAP)进行了小鼠卵母细胞和附植前胚胎端粒酶活性的测定。
- The Telomeric Repeat Amplification Protocol (TRAP) and its modified versions change the size and/or the ratio of the telomerase products in the amplification stage of the assay. 摘要：端粒酶活性直接与细胞的分生能力、生活力、生命力有着密不可分的关系， 因此对其活性测定有着重要意义。
- Methods Telomerase activity was examined in 37 brain tumor tissues and 4 normal human brain tissues by telomeric repeat amplification protocol(TRAP) assay and silver staining. 方法采用TRAP-银染法检测了37例脑肿瘤组织和4例正常脑组织中的端粒酶活性。
- Telomeric repeat amplification protocol polymerase chain reaction enzyme linked immunoadsordent assay (TRAP-PCR-ELISA) was used to examine the telomerase activity. 用端粒重复序列扩增-聚合酶链反应-酶联免疫吸附测定(TRAP-PCR-ELISA)法检测端粒酶活性的变化；
- Methods Telomerase activity was analyzed in 88 breast carcinomas specimens and 16 benign breast lesions, using PCR-based telomeric repeat amplification protocol (TRAP) assay. 方法 用建立在多聚酶链反应(PCR)基础上的端粒重复序列扩增法(TRAP)，对88例乳腺癌和16例乳腺良性疾病进行端粒酶活性检测。
- Methods: Telomerase activity was detected by Telomeric Repeat Amplification Protocol(TRAP) assay in 30 cases of the matched tumor tissues and 14 cases of tumor adjacent tissues. 方法：应用端粒重复序列扩增法，对30例食管拉网细胞标本及相应的癌组织和癌旁正常食管组织进行检测。
- The telomerase activity ofspecimens of hu man laryngealsquamous cell carcinoma ,laryngeal papilloma , vacol cord polypi, tumor surround ing mucosa and normal mucosa of vacolcord were examined by using telomeric repeat amplification protocol PCR. 方法： 采用端粒酶重复扩增?聚合酶链反应技术检测喉鳞状细胞癌、癌旁组织、喉乳头状瘤、声带息肉及正常声带粘膜的端粒酶活性。
- Method:We detected telomerase activity of HEP 2 cell line,26 cases of supraglottic carcinoma tissues and 15 cases of peri carcinoma tissues with PCR based assay designated TRAP(for telomeric repeat amplification protocol). 方法：采用端粒重复序列扩增法检测了 H E P?2 喉癌细胞系，26 例声门上型喉癌组织和15 例癌旁组织。 结果： H E P?2 喉癌细胞系呈端粒酶阳性；
- Methods The techniques of silver staining-telomeric repeat amplification protocol (TRAP), immunohistochemistry and flow cytometry (FCM) were employed to examine the telomerase activity, p53 mutation protein and SPF in 40 lung cancer specimens. 方法 采用改良银染 -端粒重复序列扩增法 (TRAP)检测 4 0例原发性支气管肺癌端粒酶活性 ,免疫组织化学方法检测其p5 3突变蛋白 ,流式细胞术检测其SPF值。
- Methods hTERT mRNA expression and telomerase activity were analyzed in 43 samples of EOC,11 benign ovarian tumors and normal ovarian tissues,as well as 4 borderline ovarian tissues with the use of RT-PCR and telomeric repeat amplification protocol(TRAP). 方法 应用RT PCR及端粒重复扩增方法对 43例上皮性卵巢癌、11例良性卵巢肿瘤和卵巢正常组织以及 4例交界性卵巢肿瘤共 69份标本进行hTERTmRNA及端粒酶活性检测。
- Methods:A plasmid including U6 promoter and siRNA of hTERT was designed and constructed. The plasmid was transfected into ALVA-41 cell line. The telomerase activity was tested by telomerase repeat amplification protocol ELISA(TRAP-ELISA). 方法:设计和构建由U6启动子驱动产生hTERT的siRNA表达质粒,转染至ALVA-41前列腺肿瘤细胞,以TRAP-ELISA方法观察细胞端粒酶活性,W estern-b lot检测端粒酶蛋白的表达,细胞记数法检测对细胞生长的影响。
- Methods Extracts of cells in surgically resected specimens and bronchoscopically biopsied specimens were analyzed for telomerase activity by the telomeric repeat amplification protocol (TRAP) assay. 方法以端粒重复扩增技术(TRAP)分析手术标本及支气管镜活检标本的细胞提取液中端粒酶的活性。TRAP分析的结果与病理检查结果进行比较。
- Methods Telomerase activity was detected in 24 primary lung cancer tissues and 6 benign neoplasms of the lungs by telomeric repeat amplification protocol (TRAP)-PCR-ELISA and TRAP-silver staining. 方法采用端粒重复扩增技术(TRAP)-PCR-ELISA定量分析及TRAP-PCR银染定性分析分法，对24例肺癌新鲜手术标本及6例肺良性肿瘤手术标本进行端粒酶活性的分析。
- Methods:Telomerase activity in 36 breast cancer and tumor-adjacent tissues,12 benign lesions and 6 normal breast tissues,was examined by telomeric repeat amplification protocol(TRAP) assay. 方法：采用端粒重复序列扩增法(telomeraic repeat amplification protocol,TRAP)来检测 36 例乳腺癌及其相应癌旁组织，12 例良性乳腺病变，6 例正常乳腺组织中的端粒酶活性。
- Methods Modified telomeric repeat amplification protocol (TRAP) was employed to examine telomerase activity in 283 malignant tumor tissuses, 42 adjacent tissuses and 61 benign tumor tissuses. 方法采用改良的银染端粒重复序列扩增方法，对283例恶性肿瘤、61例良性肿瘤和42例癌旁组织进行端粒酶活性检测。
