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- Methods Variation of P gene of HBV polymerase was determined by short segment PCR ELISA according to the recombined plasmid which was acquired by the site directed mutagensis. 方法 通过定点诱变获得的重组质粒作为标准对照 ,采用短片段PCR ELISA方法对拉米呋啶治疗的 6 0例乙肝患者血清标本进行HBV聚合酶P基因的变异检测 ,同时观察其HBVDNA定量、肝功能指标的变化。
- Secondly, we selected yeast Pichia pastoris to be the gene-expressing host and the secreting plasmid pPIC9 was as vector to construct recombined plasmid pPIC-Atmp. 研究中，我们选用巴斯德毕赤酵母作为基因表达系统，分泌型质粒pPIC9作为载体，构建了基因工程菌。
- The recombinant plasmid pUC18 E6 had been got. 获得重组质粒 pUC18 E6。
- Recombinant plasmid pGEX-Csn was transformed into E. 重组质粒pGEX-Csn转化E.
- Construction of the Mutant vp3 Gene of CAV Recombined Plasmid 鸡贫血病毒vp3突变体的构建
- The recombinant plasmid with the hammerhead ribozymegene was correct by digestion identification. 核酶基因重组子经酶切鉴定序列正确。
- Recombinant plasmid pDAlllS was constructed by inserting 1.7kb aprA into the Nrul site of aveD. 将1.;7kb安普霉素抗性基因aprA插入aveD的NruI位点，得pDA1118。
- Study on Expression of Recombinant Plasmid PsecTaq2A-AMG for Human Amelogenin in Human Gingival Fibroblast. 人釉原蛋白重组质粒Psec Taq2A-AMG在人牙龈成纤维细胞表达的研究
- Objective Construct a recombinant plasmid pET28a-EDA-EDB,prepare the fusion EDA-EDB protein. 目的构建重组pET28a-EDA-EDB质粒,制备重组纤维连接蛋白EDA-EDB融合蛋白。
- Objective To investigate the amelogenin expression of recombinant plasmid PcDNA3-AMG in COS-1 cell line. 目的研究人釉原蛋白(AMG)重组质粒PcDNA3-AMG在COS-1细胞系中的表达。
- A recombinant plasmid pUC-IL6 coding for IL6 was constructed by recombinant gene technique. 利用基因重组技术，构建含IL6基因的重组质粒pUC－IL6。
- The recombinant plasmid of antisense TIMP 1 has a certain extent reverse effects on liver fibrosis. 反义TIMP 1表达质粒对肝纤维化有一定的逆转作用
- The recombinant plasmid pBV220-Arr was construted successfully and the target protein Arresten can express in E. 成功构建Arresten基因重组质粒pBV220-Arr;并可在E.
- The recombinant plasmid pPGVT3 derived from the vector pUC4 was unstable in the E. 从载体质粒pUC4衍生的重组质粒pPGVT3在大肠杆菌宿主DF2145中是不稳定的,以pPGVT3转化DF2145时在4o℃培养得不到转化子。
- The recombinant plasmid was transformed into E. coli BL21(DE3), and E. coli BL21(DE3)/pET-LG3 was induced by IPTG. 用IPTG诱导,LG3蛋白在大肠杆菌BL21(DE3)中得到了表达,并经Ni-NTA层析柱获得纯化。
- Finally,the recombinant plasmid pGEX-4T-CTB was successfully constructed with a CTB gene fragment of 376 bps. 结果构建了重组质粒pGEX-4T-CTB,CTB基因片段分子量约为376bp;
- Authentication,PCR and DNA sequencing showed the recombinant plasmid of human MIA/CD-RAP was successfully constructed. 酶切电泳和DNA测序结果表明,成功地克隆了人黑素瘤M IA/CD-RAP cDNA。
- The Arresten gene was screening successfully,and the recombinant plasmid pBV220-Arr was constructed successful. 成功筛选出Arresten基因并构建了重组质粒pBV220-Arr,重组质粒在菌株中获得表达。
- Method The recombinant plasmid pMD18-T-gG was constructed as HSV-2 DNA standard for quantitative analysis. 方法构建重组质粒 pMD18-T-gG作为标准品。
- Methods Transform COS 7 cells with recombinant plasmid and detect instantaneously expressed product by ELISA. 方法 质粒转染COS 7细胞 ,用ELISA法测定瞬时表达产物。
