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- Recombinant retrovirus was produced by transient transfection of the Phoenix-ecotropic packaging cell line with pMX-3FLAG-Id3-EGFP vector using FuGene-6 transfection reagent. 通过脂质体转染技术将重组逆转录病毒载体(pMX-3FLAG-Id3-EGFP)转染Phoenix-ecotropic包装细胞系;
- Retrovirus mediated gene transfection was carried out. First, the package cell line PT67 was transfected by lipofectAMINE TM2000, so as to obtain recombinant retrovirus supernatant, which was directly used in the transfection of MSCs. 反转录病毒介导的基因转染,首先利用LipofectAMINETM 2000转染包装细胞系PT67,获得重组反转录病毒上清,然后用病毒上清直接感染骨髓间充质干细胞。
- recombinant retrovirus expression vector 衣壳蛋白基因
- After A549 was infected by recombinant retroviral vector expressing antisense ANG,ANG protein level decreased greatly. 构建出含反义血管生成素的逆转录病毒载体 ,感染A5 4 9细胞后 ,能有效抑制A5 4 9细胞血管生成素蛋白的表达。
- Recombinant retroviral vector expressing antisense ANG can efficiently inhibit ANG expression in A549 cell in vitro,providing the basis for further study in vivo. 利用反义血管生成素的逆转录病毒载体能够有效抑制培养细胞血管生成素的表达 ,为今后的体内应用奠定了基础。
- Objective To construct recombinant retroviral vector expressing antisense ANG and transfect it into the human lung cancer cell line A549 to inhibit the expression of ANG. 目的 构建含反义人血管生成素的真核表达载体 ,并转染人肺癌细胞株A5 4 9,使之特异性地封闭A5 4 9细胞中血管生成素的表达 ,以达到抑制肿瘤生长的目的。
- Keywords lumbrokinase;recombinant retrovirus;package;transfection;expression; 蚓激酶;重组逆转录病毒;包装;转染;表达;
- AIM: It is to investigate that apoptosis of MDA-MB-435 cell, a human breast cancer cell line, was induced by recombinant retroviral vector mediated CED-4 gene of cadherin 5 N terminal and its apoptosis mechanism. 目的:研究粘附素5 N 端CED-4(cadherin extracellular domain-4)基因经重组逆转录病毒介导转染人乳腺癌细胞MDA-MB-435 及其诱导MDA-MB-435 细胞凋亡的分子调控机制。
- Construction and Identification of recombinant retrovirus vector expressing human promyelocytic leukemia gene 急性早幼粒性白血病基因重组逆转录病毒载体构建及表达
- Construction of mIL-12 recombinant retrovirus vector and establishment of viral package cell line mIL-12重组逆转录病毒载体的构建及包装细胞系的建立
- Construction of the recombinant retrovirus vector of HBV-S gene and it's expression in eukaryotic cells 乙型肝炎病毒S基因重组逆转录病毒载体的构建及其在真核细胞中表达
- Cloning of Human Thioredoxin Gene and Construction and Identification of Its Recombinant Retrovirus Vector 人硫氧还蛋白基因克隆及其重组逆转录病毒载体的构建和鉴定
- Construction of Recombinant Retrovirus Encoded LacZ and Its Expression on NIH_3T_3 含LacZ重组逆转录病毒的制备及其在NIH_3T_3细胞的表达
- Keywords Lumbrukinase;recombinant retrovirus;Packaging;Transfection;transgene;Rabbit; 蚓激酶;重组逆转录病毒;包装;转染;转基因;
- Construction of the recombinent retroviral vector rRV-hIL-4 and expression of rheumatoid arthritis in vitro. 携带hIL-4逆转录病毒重组体的构建及在类风湿关节炎中体外表达的研究
- Recombinant retroviral expression vector 重组逆转录病毒载体
- recombinant retrovirus 重组逆转录病毒
- Retrovirus is one of the vectors in common use. 逆转录病毒是最为常用的载体之一。
- Recombinant fragments;recombinant technology. 重组DNA碎片;重组DNA技术
- The recombinant plasmid pUC18 E6 had been got. 获得重组质粒 pUC18 E6。
