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- The recombinant adenovirus is successfully constructed and can efficient express EWS/FLI1 in PBMC. 重组腺病毒AdEWS/FLI1构建成功 ,并能在PBMC中稳定有效地表达 ,为进一步进行尤文肉瘤的免疫治疗研究奠定了基础。
- Conclusion: The recombinant adenovirus generated is E1 and E3 deleted which can be further used in gene therapy in vivo. 结论：所获重组腺病毒为E1，E3缺陷型，可进一步用于基因治疗研究。
- RESULTS: The target gene of recombinant adenovirus plasmid could be amplified by PCR in different passages of HEK293 cells. 用减蛋综合征阳性血清在体外中和重组腺病毒，接种HEK293细胞；
- The HI titer of the recombinant adenovirus rAd-H1-GFP and rAd-H1-N1 immunized group was 1:243 and 1:279 respectively in 6 weeks after innoculation. 重组腺病毒rAd-H1-GFP和rAd-H1-N1对仔猪的免疫试验结果表明，免疫后2周能够检测到H1N1亚型SIV的HI抗体，免疫后6周，重组腺病毒rAd-H1-GFP免疫组的HI滴度达1：243，而重组腺病毒rAd-H1-N1免疫组的HI滴度达1：279。
- Objective:To construct a recombinant adenovirus harboring the human insulin gene regulated by the glucose-dependent insulinotropic polypeptide(GIP) promoter. 目的:构建携带葡萄糖依赖性促胰岛素多肽(GIP)启动子控制下的人胰岛素基因的腺病毒载体。
- Results: Adeno-X-SSTR2,recombinant adenovirus vector,can infect pancreatic carcinoma cells MIA-PaCa-2 and AsPC-1 efficiently,which both can express SSTR2 mRNA and their protein. 在不同浓度NC-8-12和奥曲肽作用下;未转染SSTR2基因胰腺癌细胞株的Survivin表达量无明显变化(P>0.;05);转染了SSTR2基因胰腺癌细胞株的Survivin表达量明显下调(P<0
- The infl uence of recombinant adenovirus on the growth of breast cancer cell line MCF7 was stu died. Results show that the recombinant adenovirus can inhibit the growth of MCF7. 获得的重组病毒粒子作用于人乳腺癌细胞系MCF7,初步表明重组腺病毒能抑制MCF7的生长。
- Results: The result of restriction showed the direction of the insertion was right and the recombinant adenovirus generated contained the gene of D116H-ANG. 结果：酶切结果显示，重组粘粒中，D116H-ANG插入方向正确,所获重组腺病毒带有ANG衍生物基因。
- These results show BDNF and NT3 are trophic factors of SGNs.Foregin genes mediated by recombinant adenovirus can be effectively transduced and expressed into the SGNs. 这一结果说明BDNF和NT3是听神经元的有效营养因子 ,通过腺病毒的介导外源基因可有效的转染培养的听神经元。
- Abstract:Objective:To construct the recombinant adenovirus vector carrying rat vascular endothelial growth factor (VEGF) as preparation for later use for genetic transfection. 目的:探讨构建携带大鼠血管内皮细胞生长因子(VEGF)的重组腺病毒载体方法，为后续的基因转染研究作准备。
- Objective:To construct and confirm the D116H-ANG recombinant adenovirus for the studies on gene transfection of the angiogenic factors to the ischemic cardiac muscle. 目的：构建并鉴定血管生长素(ANG)衍生物重组腺病毒,为心肌缺血区促血管生长因子基因转染研究作准备。
- The positive expressions of P16 in glioma cell line TJ905 was transmitted and colony formation of tumor cells was greatly inhibited by recombinant adenovirus, after 6 days the inhibitory rate of the growth of tumor cells was 98%. 重组体腺病毒能介导P16外源基因在恶性脑胶质瘤细胞系TJ905细胞中阳性表达,6天时肿瘤细胞生长抑制率为98%25,并且能显著地抑制肿瘤细胞的克隆形成能力。
- Objctive:It is surveyed whether the recombinant adenovirus vector pAdeno-X-siFas 1+siFas 2 expressing two Fas-targeted short hairpin RNAs(shRNA) could supress the overexpression of Fas induced by LPS/D-GalN in BALB/c mouse liver. 目的:观察串联表达Fas-shRNA的腺病毒载体pAdeno-X-siFas 1+siFas 2对脂多糖(LPS)/D-氨基半乳糖(D-GalN)诱导BALB/c鼠肝细胞Fas过表达的影响。
- Successful construction of the recombinant adenovirus vector containing the Ki67-siRNA gene was achieved and it can be used to significantly inhibit expression of the Ki67 gene and the growth human colon carcinoma cells. 含有Ki67-siRNA的重组腺病毒构建成功,其能抑制人结肠癌细胞Ki67基因表达和生长。
- Objective: To assess the treatment effect of rat C 6 glioma with recombinant adenovirus HSV tk gene (AdHCMV tk) combinated with GCV and ACV. Methods: The efficiency of AdHCMV lacZ infecting C 6 cells was studied by using x gal staining. 目的：带有HSV－tk基因的重组腺病毒（AdHCMV－tk）结合核苷类似物（NA）治疗大鼠C6脑胶质瘤。
- The recombinant adenovirus containing rPB-DR promoter and TK gene has been constructed successfully, and this lays the foundation for the research of gene therapy of prostate cancer . 成功构建出包含rPB DR启动子和TK自杀基因的重组复制缺陷型腺病毒并鉴定正确,可用于下一步前列腺癌基因治疗的研究中。
- The constructed pDC316-LMP2 was co-transfected with adenovirus backbone pBHG-fiber5/35 into 293 cells to establish the recombinant adenovirus Ad5F35-LMP2, which was confirmed by PCR and indirect immune-fluorescence test. pDC3l6-LMP2与腺病毒骨架pBHG-fiber5/35共转染293细胞构建重组腺病毒Ad5F35-LMP2,PCR及间接免疫荧光进行鉴定。
- The results of restriction and PCR showed the insertion was right and the recombinant adenovirus generated contained the CD and TK fusion gene without replication competent adenovirus. 酶切结果及 PCR结果显示 ,重组粘粒中 CD、TK融合基因插入正确 ,经鉴定所获重组腺病毒带有融合基因 ,并且无具有复制能力的腺病毒存在。
- To construct recombinant adenovirus containing the capsid(P1) and 3CD protease coding regions of O-type foot-and-mouth disease virus(FMDV),the genes of P1 and 3CD was obtained by reverse transcriptase-polymerase chain reaction(RT-PCR). 目的构建包含有O型口蹄疫病毒(FMDV)强毒结构蛋白(P1)基因及弱毒非结构蛋白(3CD)基因的重组腺病毒,为进一步研制FMD活载体疫苗奠定基础。 方法通过RT-PCR方法扩增得到含有FMDV P1、3CD编码区的目的基因。
- Methods:The HIF-dependent recombinant adenovirus Ad-5HRE/hCMVmp-BCD was constructed by DNA recombinant technique. Western blot was used to detect HIF-dependent expression of bacterial cytosine deaminase (BCD). 方法借助DNA重组技术构建HIF依赖性表达的重组腺病毒载体Ad-5HRE/hCMVmp-BCD。
